Naming core promoter elements at -10 and -35 In bacterial genes, the DNA regions located approximately at -10 and -35 relative to the transcription start site are called:
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AStart codons
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BoriC regions
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CShine–Dalgarno sequences
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DPromoters
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ETelomeres
Answer
Correct Answer: Promoters
Explanation
Introduction / Context:Bacterial transcription initiation relies on conserved promoter motifs. Recognizing these helps distinguish transcriptional from translational signals.
Given Data / Assumptions:
- -35 and -10 elements are characteristic promoter regions.
- They are recognized by sigma factor within the RNA polymerase holoenzyme.
Concept / Approach:The -10 (Pribnow) and -35 hexamers define the core promoter in many bacterial genes, positioning polymerase and facilitating DNA melting. They are not translation or replication elements.
Step-by-Step Solution:Identify the location: upstream of the transcription start site at defined negative positions.Associate those with promoter function and sigma recognition.Choose “Promoters.”
Verification / Alternative check:Mutational changes to these motifs reduce initiation rate; compensatory sigma mutations can restore recognition, confirming promoter identity.
Why Other Options Are Wrong:
- Start codons / Shine–Dalgarno: Translation signals on mRNA, not DNA promoter motifs.
- oriC: The chromosomal origin of replication, unrelated to individual gene promoters.
- Telomeres: Eukaryotic chromosome ends, absent in bacteria.
Common Pitfalls:Confusing transcription initiation signals with translation initiation sequences.
Final Answer:Promoters.