Enteric selection: Intestinal (enteric) bacteria typically grow in the presence of which inhibitory additive, whereas many nonintestinal bacteria are suppressed by it?
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Abile salts
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Blow concentration of various dyes
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Csugars
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Dlow levels of nitrogen
Answer
Correct Answer: bile salts
Explanation
Introduction / Context:Selective media exploit physiological differences to favor target organisms. Enteric bacteria are adapted to the gut environment and can tolerate compounds that inhibit nonenteric flora, improving diagnostic recovery.
Given Data / Assumptions:
- We compare additives frequently used in selective media.
- Bile salts are common in enteric-selective formulations.
- Goal: identify the additive that enterics tolerate but others do not.
Concept / Approach:
Bile salts mimic intestinal conditions and are incorporated in media such as MacConkey and SS agars to suppress Gram-positive organisms and many nonenterics while allowing Gram-negative enteric rods to grow. This enhances isolation of pathogens like Salmonella and Shigella from stool specimens.
Step-by-Step Solution:
Identify the gut-mimicking inhibitor: bile salts.Recall that enterics possess outer membrane and efflux systems conferring tolerance.Exclude options that are not inherently selective for enterics (e.g., sugars).Select “bile salts.”Verification / Alternative check:
Formulations like MacConkey agar list bile salts among selective agents; growth patterns in labs consistently show enteric Gram-negatives thriving while Gram-positives are inhibited.
Why Other Options Are Wrong:
Low concentration of dyes: while some dyes are selective (e.g., crystal violet), bile salts are the hallmark for enterics.
Sugars and low nitrogen: these are nutrients or conditions, not primary selective inhibitors for enterics.
Common Pitfalls:
Assuming any dye equals enteric selectivity; overlooking that bile salts specifically emulate intestinal bile, a natural barrier many enterics tolerate.
Final Answer:
bile salts