Reporting viable plate counts In bacteriology reports, colony counts from viable plate methods are most commonly expressed in which unit?
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Acfu (no volume specified)
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Bcfu/mL (colony-forming units per milliliter)
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CBoth cfu and cfu/mL are standard for the same test
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Dthousands/mL
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EPercent growth per plate
Answer
Correct Answer: cfu/mL (colony-forming units per milliliter)
Explanation
Introduction / Context:Standardized reporting ensures clinicians can interpret results consistently across laboratories. Viable counts typically quantify organisms relative to sample volume to allow comparisons across patients and time.
Given Data / Assumptions:
- Plate counts originate from a measured volume plated after serial dilution.
- Clinical thresholds (for example, urinary infection cutoffs) are defined in CFU per milliliter.
- Results should allow direct comparison independent of plating volume.
Concept / Approach:The conventional unit is CFU per mL (cfu/mL). Reporting only “cfu” lacks context because colony numbers depend on the volume plated and dilution factor. Expressing thousands/mL is nonstandard and may obscure significant digits; percent growth is meaningless for plate counts.
Step-by-Step Solution:Count colonies on a plate within 30–300 colonies.Compute CFU/mL = colonies * (1 / plated volume in mL) * dilution factor.Report the final value as cfu/mL (for example, 1.6 × 10^5 cfu/mL).Round and flag according to clinical thresholds if applicable.
Verification / Alternative check:Laboratory accreditation guidelines and clinical microbiology texts use cfu/mL for urine, respiratory specimens, and quantitative wound cultures.
Why Other Options Are Wrong:
- A: “cfu” alone omits the critical volume context.
- C: Using both simultaneously for the same test would be inconsistent; cfu/mL is preferred.
- D: “thousands/mL” is an informal expression and not standard.
- E: “Percent growth” is not a recognized unit for plate counts.
Common Pitfalls:Forgetting to include the dilution factor or plated volume; misreporting significant figures beyond the method’s precision.
Final Answer:cfu/mL (colony-forming units per milliliter)