Enriched culture media: Which of the following are important examples of enriched media used to support fastidious organisms?

Difficulty: Easy

Correct Answer: All of these

Explanation:


Introduction / Context:
Enriched media contain additional nutrients (e.g., blood, serum, growth factors) to support the growth of fastidious bacteria that cannot grow on simple basal media. Recognizing examples helps select the right medium for diagnostic isolation.



Given Data / Assumptions:

  • Loeffler’s serum slope, Bordet–Gengou, and blood agar are listed.
  • All three are classically used for fastidious pathogens.
  • Enriched media are distinct from selective and differential media, though some can be both enriched and selective.


Concept / Approach:

Loeffler’s serum slope enhances growth of Corynebacterium diphtheriae and accentuates metachromatic granules. Bordet–Gengou medium, containing potato infusion and blood, supports Bordetella pertussis. Blood agar (5–10% defibrinated blood) supports many fastidious organisms and allows observation of hemolysis patterns.



Step-by-Step Solution:

Identify each listed medium as enriched by virtue of added serum or blood.Note their specific clinical use cases.Conclude that all listed examples are enriched media.Select “All of these.”


Verification / Alternative check:

Diagnostic laboratory references categorize these as enriched media; blood agar also functions as differential based on hemolysis but remains enriched due to blood content.



Why Other Options Are Wrong:

Choosing any single medium ignores other valid enriched examples present.

Excluding any would contradict standard microbiology curricula.



Common Pitfalls:

Confusing enriched with selective; some enriched media (e.g., chocolate agar with antibiotics) can be both. Always separate the purpose: enrichment supplies nutrients; selection inhibits competitors.



Final Answer:

All of these

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