Central dogma — What is the primary role of messenger RNA (mRNA) in gene expression?

Difficulty: Easy

Correct Answer: It is a direct copy of a gene

Explanation:


Introduction:
Gene expression involves transcription of DNA into RNA and translation of RNA into protein. This question identifies the specific function of messenger RNA within that flow of information.


Given Data / Assumptions:

  • DNA encodes genes.
  • RNA species include mRNA, rRNA, and tRNA with distinct roles.
  • Protein synthesis occurs on ribosomes using mRNA templates.


Concept / Approach:
mRNA is synthesized by RNA polymerase as a complementary RNA copy of the coding region of a gene (using the template strand). After processing (5′ cap, splicing, poly(A) tail) in eukaryotes, the mature mRNA carries the codon sequence that directs amino acid order during translation.


Step-by-Step Solution:

Transcription: DNA → pre-mRNA → processed mRNA.Export and translation: mRNA binds ribosomes; tRNAs decode codons into amino acids.Therefore, mRNA functions as the direct genetic message specifying the protein sequence.Other RNAs provide structural or adaptor roles, not the primary message content.


Verification / Alternative check:
mRNA sequencing (RNA-seq) reads out expressed gene copies, and in vitro translation using purified mRNA demonstrates that its sequence alone encodes the protein product.


Why Other Options Are Wrong:

  • Carries amino acids: tRNA, not mRNA, carries amino acids as aminoacyl-tRNA.
  • Ribosome component: rRNA and ribosomal proteins form the ribosome.
  • Genetic material of some organisms: RNA genomes exist in some viruses, but that is not the role of cellular mRNA.
  • Catalyzes peptide bond formation: peptidyl transferase activity resides in rRNA within the large subunit.


Common Pitfalls:
Confusing the roles of different RNA classes during translation; mRNA is the template, tRNA is the adaptor, and rRNA is the catalytic/structural core.


Final Answer:
It is a direct copy of a gene.

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