Difficulty: Easy
Correct Answer: Transposons with transposase/integrase functions
Explanation:
Introduction / Context:
Integration of mobile DNA into chromosomes underlies genome plasticity, antibiotic resistance spread, and genetic engineering tools. Elements differ in whether they encode the machinery for movement.
Given Data / Assumptions:
Concept / Approach:
By definition, a transposon is a segment of DNA able to move to new genomic sites, typically flanked by inverted repeats. Its enzyme(s) catalyze excision/insertion or replicative transposition into the host chromosome or plasmids.
Step-by-Step Solution:
Identify which element has dedicated integration machinery: transposon.Exclude generic replicons and plasmids that may not encode such functions.Reject nonexistent categories.
Verification / Alternative check:
Experimental mutagenesis using transposons (for example, Tn5, Tn10) relies on their encoded transposase for chromosomal insertions.
Why Other Options Are Wrong:
Common Pitfalls:
Equating “plasmid” with “integrative plasmid”; only specialized elements carry integration genes.
Final Answer:
Transposons with transposase/integrase functions
Discussion & Comments