Difficulty: Easy
Correct Answer: Competent
Explanation:
Introduction / Context:
Transformation is the uptake of free DNA by bacteria. Only cells in a specific physiological state—called competence—can bind, import, and integrate extracellular DNA. Competence may occur naturally (e.g., Streptococcus pneumoniae, Bacillus subtilis) or be induced artificially in the lab (chemical competence or electroporation).
Given Data / Assumptions:
Concept / Approach:
Competent cells express surface structures and DNA transport machinery that facilitate binding and translocation of DNA across the membrane(s). Once inside, DNA can recombine with the chromosome or persist as a plasmid (if replication origin is compatible).
Step-by-Step Solution:
Verification / Alternative check:
Laboratory protocols routinely create competent E. coli cells (e.g., CaCl2 or electroporation), validating the terminology.
Why Other Options Are Wrong:
Common Pitfalls:
Final Answer:
Competent
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