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Home Biochemical Engineering Agitation and Aeration Comments

  • Question
  • During fermentation, the saturation concentration of oxygen was reduced from 7 ppm. to 1 ppm. Which one of the following conclusions can be drawn?


  • Options
  • A. Microbial growth is oxygen limited since the oxygen concentration is lesser than the saturation concentration
  • B. Microbial growth is not oxygen limited since the oxygen concentration is lesser than the saturation concentration
  • C. Experimental error leads to the reduction
  • D. No conclusion can be drawn

  • Correct Answer
  • No conclusion can be drawn 


  • Agitation and Aeration problems


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    • 1. For aerated mixing vessels in an aqueous solution, the mass transfer coefficient (kL) co-relating power consumption (Pm) and fermenter volume (V) can be expressed as

    • Options
    • A. kL ? (Pm/V)0.33
    • B. kL ? (Pm/V)0.66
    • C. kL ? (Pm/V)0.50
    • D. kL ? (Pm/V)
    • Discuss
    • 2. Flooding is a condition/phenomenon which occur in a bioreactor when

    • Options
    • A. The air escapes without being distributed by the agitator
    • B. The fermenter medium spills out the reactor due to foaming
    • C. The volume of foam is more than the volume of broth
    • D. None of the above
    • Discuss
    • 3. Which of the following is correct for the off-centre impellers?

    • Options
    • A. Off-centre impellers can be used with any size reactor
    • B. Off-centre impellers should only be used with reactors with volumes less than 10 m3
    • C. The impeller should never be placed in an off-centre position
    • D. Off-centre impellers can be used when volume is greater than 10 m3
    • Discuss
    • 4. A cylindrical reactor fitted with Rushton Turbine has a liquid volume of 100,000 litres. It has an aspect ratio (the ratio of the channel height to the width) of 2:1. The diameter of the impeller should be approximately:

    • Options
    • A. 1.3 m
    • B. 3.3 m
    • C. 5.3 m
    • D. 7.3 m
    • Discuss
    • 5. Pure oxygen, if used instead of air will increase oxygen transfer rates because

    • Options
    • A. the saturation concentration of oxygen is higher
    • B. the bubble size is smaller
    • C. the oxygen transfer coefficient is larger
    • D. the partial pressure of oxygen in the gas phase is lower
    • Discuss
    • 6. A fermentation system has a KLa of 3 s-1 and a C0* of 5 ppm of O2. If the bulk liquid is completely depleted of oxygen, then the oxygen transfer rate will be equal to

    • Options
    • A. zero
    • B. 15 mg.l-1.s-1
    • C. 10 mg.l-1.s-1
    • D. 5 mg.l-1.s-1
    • Discuss
    • 7. Impeller damage can be minimized by

    • Options
    • A. using specially designed axial flow impellers
    • B. using radial flow impellers
    • C. using Intermig impellers
    • D. all of the above
    • Discuss
    • 8. In aerobic microorganisms cultures radial flow impellers are used because

    • Options
    • A. they provide energy efficient high turbulent mixing conditions
    • B. they provide low shear mixing conditions
    • C. they provide high shear mixing conditions
    • D. they ensure that bubble size is maximized leading to high oxygen transfer rates
    • Discuss
    • 9. A fermentation system has a KLa of 3 s-1 and a C0* of 5 ppm of O2. If the bulk liquid is saturated with oxygen then the oxygen transfer rate will be equal to

    • Options
    • A. zero
    • B. 3 mg.l-1.s-1
    • C. 5 mg.l-1.s-1
    • D. 15 mg.l-1.s-1
    • Discuss
    • 10. If the stirrer speed is too slow, bubbles will accumulate and coalescese under the impeller. This phenomenon is known as a

    • Options
    • A. coalesced impeller
    • B. flooded impeller
    • C. river impeller
    • D. dry impeller
    • Discuss


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