In aerobic microbial cultures, why are radial-flow impellers (e.g., Rushton turbines) commonly employed?
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AThey provide energy-efficient, low-turbulence mixing
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BThey provide low-shear mixing conditions
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CThey provide high-shear, highly turbulent mixing that enhances gas dispersion
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DThey maximize bubble size to raise oxygen transfer rates
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EThey eliminate the need for baffles
Answer
Correct Answer: They provide high-shear, highly turbulent mixing that enhances gas dispersion
Explanation
Introduction / Context:Choosing the right impeller for aerobic fermentation is central to achieving adequate oxygen transfer. Radial-flow impellers such as the Rushton turbine are classics in bioprocessing because they generate intense local turbulence and strong gas dispersion, raising interfacial area and kLa under many conditions.
Given Data / Assumptions:
- Aerated, baffled vessel with mechanical agitation.
- Objective is maximizing oxygen transfer and mixing.
- Media viscosity is moderate.
Concept / Approach:Radial-flow turbines discharge flow laterally into baffles, creating high energy dissipation zones. The resulting shear breaks bubbles into smaller sizes, increasing interfacial area and improving dispersion uniformity, which typically elevates kLa compared to gentle, low-shear devices.
Step-by-Step Solution:
Identify hydrodynamic signature: strong radial jets and high shear near blades.Connect to mass transfer: smaller bubbles and vigorous circulation raise kLa.Conclude that the purpose is high-shear, turbulence-driven dispersion.Choose the statement reflecting this function.Verification / Alternative check:kLa correlations often show higher exponents on power input for Rushton turbines versus some axial designs in particular regimes, corroborating their dispersion strength.
Why Other Options Are Wrong:
- Energy-efficient low turbulence/low shear: describes axial hydrofoils, not radial turbines.
- Maximize bubble size: the opposite; they reduce bubble size.
- Eliminate baffles: baffles are still recommended to prevent swirl.
Common Pitfalls:Assuming high shear is always beneficial; for shear-sensitive cells, axial-flow alternatives may be preferable.
Final Answer:They provide high-shear, highly turbulent mixing that enhances gas dispersion