Biosurfactants in fermentation: detergent-like substances produced by microbes during culture will typically cause which interfacial effects in an aerated bioreactor?

Difficulty: Easy

Correct Answer: All of the above

Explanation:


Introduction / Context:
Many microbes secrete surface-active molecules (biosurfactants) such as rhamnolipids or proteins that alter gas–liquid interfacial properties. These changes can strongly influence foam formation, bubble size distributions, oxygen transfer, and antifoam requirements.


Given Data / Assumptions:

  • Aerated, agitated culture with measurable foam formation.
  • Surface-active compounds present at concentrations near or above their critical micelle concentration.
  • No antifoam initially added.


Concept / Approach:
Surfactants preferentially adsorb at interfaces, lowering surface tension. Lower surface tension and interfacial elasticity stabilize small bubbles and suppress coalescence, increasing gas holdup and often promoting foam stability. While this can improve interfacial area, it may reduce effective kLa if excessive foaming disrupts gas handling or causes flooding.


Step-by-Step Solution:

Recognize adsorption at the gas–liquid interface as the primary effect.Infer decreased surface tension from surfactant presence.Conclude reduced bubble coalescence and increased foam stability follow.


Verification / Alternative check:
Surface tension measurements (du Nouy ring, Wilhelmy plate) and bubble column tests show decreased σ and altered bubble size distribution in the presence of biosurfactants.


Why Other Options Are Wrong:

  • “None” contradicts well-documented surfactant behavior.


Common Pitfalls:
Assuming lower surface tension always increases kLa; excessive foaming can hinder mass transfer and operations.


Final Answer:
All of the above.

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