Choose the correct statements about the cell wall of Gram-positive bacteria (structure and chemistry):

Introduction / Context:
Gram-positive and Gram-negative bacteria differ markedly in their cell envelope architecture. Identifying key features of the Gram-positive wall helps explain staining behavior, susceptibility to antibiotics, and interactions with the host immune system.

Given Data / Assumptions:

• Gram-positive bacteria lack an outer membrane and instead feature a thick peptidoglycan layer.
• Teichoic acids (wall teichoic acids and lipoteichoic acids) are characteristic components of Gram-positive walls.
• “Multiple layers” here refers to stacked peptidoglycan sheets creating substantial thickness.

Concept / Approach:
All listed statements accurately describe Gram-positive walls. The thick, multilayered peptidoglycan allows retention of the crystal violet–iodine complex during Gram staining. Teichoic acids contribute to cell wall maintenance, ion homeostasis, and antigenicity.

Step-by-Step Solution:

Verification / Alternative check:
Electron microscopy shows a thick, homogenous wall in Gram-positives; biochemical extraction identifies teichoic acids unique to this group.

Why Other Options Are Wrong:
Each individual statement is correct; selecting only one would omit other true features. Thus, the comprehensive option is best.

Common Pitfalls:
Confusing teichoic acids (Gram-positive) with lipopolysaccharide (LPS), which is exclusive to the outer membrane of Gram-negative bacteria.

Final Answer:
All of these