Staphylococcal haemolysins – action on human red blood cells Which staphylococcal haemolysin is noted for not causing lysis of human red blood cells (RBCs) under standard conditions?

Introduction / Context:
Staphylococcus aureus produces several cytolysins (α, β, γ, δ) with differing target specificities. Understanding their activity aids interpretation of hemolysis patterns and correlates with pathogenic potential.

Given Data / Assumptions:

• Different haemolysins act via distinct mechanisms (pore formation vs. enzymatic sphingomyelinase).
• Hemolysis is often studied using animal RBCs; species susceptibility varies.
• Human RBCs show differential sensitivity to these toxins.

Concept / Approach:
α haemolysin (alpha-toxin) forms pores and readily lyses human and rabbit RBCs. γ haemolysin is a bicomponent toxin targeting leukocytes but can lyse RBCs of various species under some conditions. δ haemolysin is a small peptide with broad but relatively weak cytolytic activity. β haemolysin (sphingomyelinase C) shows pronounced “hot–cold” hemolysis, especially of sheep RBCs, but it is notably ineffective against human RBCs due to low sphingomyelin content and membrane composition differences.

Step-by-Step Solution:
Review each haemolysin’s mechanism and species tropism.Note β haemolysin’s poor lysis of human RBCs versus strong effect on sheep RBCs.Select “β haemolysin.”

Verification / Alternative check:
Laboratory demonstrations classically use sheep blood agar to visualize β haemolysin hot–cold hemolysis; comparable lysis is not seen with human RBCs.

Why Other Options Are Wrong:

• α haemolysin: efficiently lyses human RBCs by pore formation.
• γ haemolysin: can lyse RBCs and leukocytes, depending on conditions.
• δ haemolysin: exhibits broad cytolysis; not specifically non-hemolytic for human RBCs.
• PVL: leukocidin targeting neutrophils rather than RBCs; the question focuses on classic haemolysins.

Common Pitfalls:
Assuming all hemolysins behave similarly across species; membrane lipid composition drives susceptibility.

Final Answer:
β haemolysin.