Typical features of Staphylococcus aureus on routine media: Most strains of S. aureus demonstrate which combination of laboratory characteristics?

Introduction / Context:
Recognizing hallmark traits of Staphylococcus aureus supports rapid presumptive identification before definitive tests return. Pigmentation, enzyme production, and hemolysis collectively paint a characteristic laboratory picture.

Given Data / Assumptions:

• Observation is on standard media such as sheep blood agar.
• “Most strains” implies common but not absolute traits.
• We consider phosphatase activity, golden pigment, and β-hemolysis.

Concept / Approach:
S. aureus often produces a golden carotenoid pigment (staphyloxanthin), shows β-hemolysis on sheep blood agar (though intensity can vary), and produces phosphatase. Taken together, these features are frequently present and help distinguish it from coagulase-negative staphylococci.

Step-by-Step Solution:
Assess pigment: golden-yellow colonies are typical of S. aureus. Assess hemolysis: many strains exhibit β-hemolysis. Assess enzymes: phosphatase production is common. Therefore, select the inclusive option “all of the above.”

Verification / Alternative check:
Parallel coagulase positivity strongly supports S. aureus when combined with these traits; MALDI-TOF or molecular assays can confirm.

Why Other Options Are Wrong:
Each single-feature option is incomplete; multiple typical features coexist in most strains.

Common Pitfalls:
Expecting every isolate to be strongly β-hemolytic or deeply pigmented; expression can vary with medium and incubation conditions.

Final Answer:
all of the above