Which genetic element intrinsically carries genes needed for its own integration into a host chromosome?
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AReplicon (any autonomously replicating DNA)
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BPlasmids lacking integration machinery
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CTransposons with transposase/integrase functions
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D“Tandons” (nonexistent element)
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EHousekeeping operons
Answer
Correct Answer: Transposons with transposase/integrase functions
Explanation
Introduction / Context:Integration of mobile DNA into chromosomes underlies genome plasticity, antibiotic resistance spread, and genetic engineering tools. Elements differ in whether they encode the machinery for movement.
Given Data / Assumptions:
- Transposons encode transposase (and often accessory factors) enabling cut-and-paste or copy-and-paste movement.
- Many plasmids do not encode integration systems; some can integrate via recombination but not all carry integrases.
- “Tandons” is not a recognized genetic element.
Concept / Approach:By definition, a transposon is a segment of DNA able to move to new genomic sites, typically flanked by inverted repeats. Its enzyme(s) catalyze excision/insertion or replicative transposition into the host chromosome or plasmids.
Step-by-Step Solution:Identify which element has dedicated integration machinery: transposon.Exclude generic replicons and plasmids that may not encode such functions.Reject nonexistent categories.
Verification / Alternative check:Experimental mutagenesis using transposons (for example, Tn5, Tn10) relies on their encoded transposase for chromosomal insertions.
Why Other Options Are Wrong:
- Replicon: replication competency does not imply mobility/integration.
- Plasmids: many are extrachromosomal only unless aided by recombination.
- “Tandons” and housekeeping operons: not mobile elements.
Common Pitfalls:Equating “plasmid” with “integrative plasmid”; only specialized elements carry integration genes.
Final Answer:Transposons with transposase/integrase functions