Clonal propagation—Which of the following are disadvantages of conventional (non-tissue-culture) methods compared with plant tissue culture? Consider how traditional nursery practices fare versus in vitro micropropagation in terms of speed, hygiene, storage, and logistics. Identify the combination that correctly lists disadvantages of conventional approaches for large-scale clonal multiplication.

Difficulty: Easy

Correct Answer: Both (b) and (c)

Explanation:


Introduction / Context:
Clonal propagation aims to multiply genetically uniform, high-performing plants. Conventional nursery methods (cuttings, layers, divisions) work, but they are slower and more exposed to pests, pathogens, and environmental constraints than plant tissue culture. This question asks you to pinpoint the disadvantages of conventional methods when compared with modern micropropagation.



Given Data / Assumptions:

  • Goal is rapid, large-scale clonal multiplication.
  • Conventional methods rely on field or greenhouse conditions.
  • Micropropagation (in vitro) offers asepsis, year-round production, and compact storage/transport.


Concept / Approach:
Identify which statements truly describe drawbacks of conventional (non-tissue-culture) propagation relative to micropropagation. Focus on disease status, multiplication rate, and logistics (storage/transport of planting material).



Step-by-Step Solution:

Check option (b): Conventional methods often have lower multiplication rates of disease-free plants because stock and environment are harder to sterilize and maintain pathogen-free.Check option (c): Conventional propagules (bulky cuttings, rooted plants) are heavier, require more space, and are harder to store/ship than small, clean in vitro plantlets.Option (a) is not a disadvantage of conventional methods; “multiplication of sexually derived sterile hybrids” is actually an advantage of micropropagation, not a negative for conventional methods.Therefore, the correct combined choice is (b) and (c).


Verification / Alternative check:
Commercial protocols for bananas, ornamentals, and forest trees favor micropropagation to achieve disease indexing, phytosanitary movement, and compact shipping—advantages that directly counter the limitations of conventional propagation.



Why Other Options Are Wrong:

  • (a) Misframed and not a disadvantage; sterile hybrids are better managed via tissue culture.
  • (e) Incorrect because (b) and (c) are genuine drawbacks of conventional propagation.


Common Pitfalls:
Confusing “what tissue culture can do” with “what conventional methods lack.” Also, overlooking transport/storage economics when scaling nurseries.



Final Answer:
Both (b) and (c)

More Questions from Plant Cell Culture

Discussion & Comments

No comments yet. Be the first to comment!
Join Discussion