Callus competence—What is the term for the ability of callus cells to regenerate a whole plant? Choose the precise biological term used in plant tissue culture for the capacity of component cells in a callus to form an entire plant under appropriate conditions.
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ATotipotency
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BRedifferentiation
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CDedifferentiation
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DSomaclonal variation
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ETransdifferentiation
Answer
Correct Answer: Totipotency
Explanation
Introduction / Context:Plant tissue culture exploits the remarkable capacity of somatic cells to regenerate whole plants. The correct term for this inherent ability is “totipotency,” a foundational idea that underlies micropropagation, somatic embryogenesis, and organogenesis.
Given Data / Assumptions:
- Callus cells can be induced to form shoots, roots, or somatic embryos.
- Appropriate hormonal regimes (auxin/cytokinin balance) and media are provided.
- Regenerated plantlets are genetically similar to the donor (barring somaclonal variation).
Concept / Approach:Define and distinguish related terms. Totipotency is the potential to regenerate an entire organism. Dedifferentiation refers to the reversion of specialized cells to a meristematic state (often forming callus). Redifferentiation is the subsequent specialization into organs or embryos. Somaclonal variation denotes genetic/epigenetic changes arising during culture.
Step-by-Step Solution:
Identify the capability described: whole-plant regeneration from callus cells.Map that capability to the correct term: totipotency.Eliminate similar but distinct processes (de- and redifferentiation) and unrelated terms.Verification / Alternative check:Classic examples include regeneration from tobacco and carrot callus; single cells or small aggregates can yield complete, fertile plants.
Why Other Options Are Wrong:
- Redifferentiation/dedifferentiation describe steps within regeneration, not the overarching capacity.
- Somaclonal variation is a by-product, not a capacity.
- Transdifferentiation is not the standard term in this context.
Common Pitfalls:Using “redifferentiation” as a synonym for totipotency; they are not interchangeable.
Final Answer:Totipotency