Fermentation Kinetics problems
- 1. When intracellular enzymes of whole cells are to be used in a bio-conversion process, it is often necessary to____________the cells.
Options- A. permeabilize
- B. lyophilize
- C. heat-kill
- D. denature Discuss
Correct Answer: permeabilize
- 2. The region of an airlift bioreactor in which the liquid travels in a downward direction is called the
Options- A. downcomer
- B. disengagement zone
- C. air riser
- D. flotsam Discuss
Correct Answer: downcomer
- 3. The function of the disengagement zone in an airlift fermenter is to
Options- A. prevent CO2 rich bubbles from entering the downcomer
- B. reduce the velocity of the bubbles
- C. reduce liquid loss as aerosols
- D. all of the above Discuss
Correct Answer: all of the above
- 4. Chemostats work on the principle of
Options- A. maintaining constant volume of culture medium
- B. maintaining continuous flow of nutrients
- C. maintaining uniform nutrients concentration
- D. operating at higher pressure Discuss
Correct Answer: maintaining uniform nutrients concentration
- 5. Population doubling time, td can be expressed as (where ? is the specific growth rate.)
Options- A. log2/?
- B. ln2/?
- C. ?/ln2
- D. ?/log2 Discuss
Correct Answer: ln2/?
- 6. The monod model predicts that the specific growth rate
Options- A. will increase with the concentration of the growth limiting substrate until it reaches a maximum value
- B. will decrease with the concentration of the growth limiting substrate
- C. will increase with the concentration of the growth limiting substrate
- D. does not depend on growth limiting substrate Discuss
Correct Answer: will increase with the concentration of the growth limiting substrate until it reaches a maximum value
- 7. The specific growth rate (?) is defined as
Options- A. the concentration of biomass in the reactor
- B. rate of increase of total biomass in a reactor
- C. the rate of individual cells division or increase in their biomass
- D. the rate of cell death Discuss
Correct Answer: the rate of individual cells division or increase in their biomass
- 8. During the exponential phase the maximum specific growth rate equals specific growth rate as
Options- A. concentration of the growth limiting substrate is much less than the monod constant
- B. concentration of growth limiting substrate is much greater than the monod constant
- C. specific growth rate increases exponentially
- D. concentration of the growth limiting substrate is equal to the monod constant Discuss
Correct Answer: concentration of growth limiting substrate is much greater than the monod constant
- 9. The function of a mechanical seal is to
Options- A. prevent contaminants entering the reactor
- B. prevent cells from leaving the reactor
- C. both (a) and (b)
- D. prevent air to enter Discuss
Correct Answer: both (a) and (b)
- 10. The Monod Model relates
Options- A. substrate utilized with the biomass consumption
- B. specific growth rate to the substrate availability
- C. yield with the biomass utilization
- D. the biomass concentration with specific growth rate Discuss
Correct Answer: specific growth rate to the substrate availability
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