Enzymes and Kinetics problems
- 1. The Woolf-Augusteinsson-Hofstee plot of ν versus ν/[S] and the Eadie-Scatchard plot of ν/[S] versus ν do not involve reciprocals of ν therefore are considered to be more reliable when the error in v is
Options- A. non-significant
- B. significant
- C. nothing to do with the reliability
- D. non significant in selected cases Discuss
Correct Answer: significant
- 2. A noncompetitive inhibitor of an enzyme-catalyzed reaction
Options- A. increases KM and increases Vmax
- B. increases KM and reduces Vmax
- C. reduces KM and increases Vmax
- D. reduces KM and reduces Vmax Discuss
Correct Answer: increases KM and reduces Vmax
- 3. Quasi steady state is also known as
Options- A. Michaelis Menten approach
- B. Briggs-Haldane approach
- C. Pseudo steady state
- D. all of the above Discuss
Correct Answer: Pseudo steady state
- 4. The plot commonly used for determining the value of Vmax is
Options- A. Lineweaver Burk plot
- B. Langmuir plot
- C. Eadie Hofstee plot
- D. all of these Discuss
Correct Answer: all of these
- 5. An allosteric inhibitor of an enzyme usually
Options- A. participates in feedback regulation
- B. denatures the enzyme
- C. is a hydrophobic compound
- D. causes the enzyme to work faster Discuss
Correct Answer: participates in feedback regulation
- 6. In competitive inhibition a factor is obtained from the measurement of
Options- A. Vmax
- B. KM
- C. Y-intercept in Lineweaver-Burk Plot
- D. None of these Discuss
Correct Answer: KM
- 7. The reciprocal equation for non competitive inhibition can be arranged to the equation for the
Options- A. Dixon plot
- B. Woolf-Augusteinsson-Hofstee plot
- C. Eadie-Scatchard plot
- D. Hanes-Woolf plot Discuss
Correct Answer: Dixon plot
- 8. The relationship between Keq, Km and Vmax is known as
Options- A. Haldane equation
- B. Michaelis Menten equation
- C. Numerical solution approach
- D. Gibbs-Helmholtz equation Discuss
Correct Answer: Haldane equation
- 9. The types of inhibition pattern based on Michaelis Menten equation are
Options- A. competitive
- B. non-competitive
- C. uncompetitive
- D. all of the above Discuss
Correct Answer: all of the above
- 10. Which of the following activity is possible by transferases?
Options- A. Transfer of methyl groups
- B. Transfer of glycosyl group
- C. Both (a) and (b)
- D. None of these Discuss
Correct Answer: Both (a) and (b)
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