Fermentation Kinetics problems
- 1. Why a T-flask used in small-scale cell culture is incubated in a horizontal position?
Options- A. To save space
- B. To increase the surface area of the liquid-air interface
- C. Both (a) and (b)
- D. To increase the rate of oxygen transfer into the liquid Discuss
Correct Answer: Both (a) and (b)
- 2. Wash out in steady state fermentation occurs when
Options- A. dilution rate is less than maximum specific growth rate
- B. dilution rate is higher than the maximum specific growth rate
- C. cell concentration reaches the maximum
- D. specific growth rate is maximum Discuss
Correct Answer: dilution rate is higher than the maximum specific growth rate
- 3. Which of the following is not correct for the Monod model and the Michaelis Menten Model?
Options- A. The Michaelis Menten Model was derived from a curve fitting exercise
- B. The Michaelis Menten model was derived from an analysis of the mechanism of microbial growth
- C. The Monod model was derived from an analysis of the mechanism of microbial growth
- D. All of the above Discuss
Correct Answer: The Monod model was derived from an analysis of the mechanism of microbial growth
- 4. In the accelerated phase, cell starts to
Options- A. increase and the division rate increases to reach a maximum
- B. decrease and the division rate increases to reach a maximum
- C. increase and the division rate decreases to reach a maximum
- D. increase and the division rate increases to reach a minimum Discuss
Correct Answer: increase and the division rate increases to reach a maximum
- 5. An open system in which the growth rate is maintained by adding a nutrient (present in limiting quantities) at the same rate as that medium containing micro-organisms is removed is called
Options- A. manostat
- B. chemostat
- C. turbidostat
- D. culturostat Discuss
Correct Answer: chemostat
- 6. The height to diameter ratio (H/D) for the column fermenters is
Options- A. <3
- B. >3
- C. <1.5
- D. >1.5 Discuss
Correct Answer: >3
- 7. In the stationary phase
Options- A. growth is proportional to death
- B. growth rate is equal to death rate
- C. growth rate is lesser than death rate
- D. no co-relation exist between death rate and growth rate Discuss
Correct Answer: growth rate is equal to death rate
- 8. Biomass concentrations during fermentation is
Options- A. diluting the samples to optical density less than 0.3
- B. monitored by controlling the changes in biomass concentrations
- C. considering that optical density is proportional to the surface area of the biomass in the sample
- D. all of the above Discuss
Correct Answer: all of the above
- 9. The dilution rate, D is defined as(where F = volumetric flow rate, VR = total volume of culture in the reactor and ? specific growth rate)
Options- A. F/VR
- B. VR/F
- C. ?/F
- D. F/? Discuss
Correct Answer: F/VR
- 10. Which of the following is an example of a mathematical model?
Options- A. Volume of cuboid = length x breadth x height
- B. The Monod Model
- C. The Michaelis Menten Equation
- D. All of the above Discuss
Correct Answer: All of the above
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