Immobilized Enzyme problems
- 1. Which is not the method for producing immobilized enzymes with multifunctional reagents?
Options- A. Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
- B. Functional groups are introduced on the support to react co-valently with enzymes
- C. Enzymes are cross linked intermolecularly
- D. None of the above Discuss
Correct Answer: None of the above
- 2. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
Options- A. copolymerization or microencapsulation
- B. crosslinking using multifunctional reagents
- C. adsorption
- D. all of the above Discuss
Correct Answer: copolymerization or microencapsulation
- 3. The support material for immobilization of cells of Bacillus subtilis is
Options- A. ion exchange resins
- B. gelatin
- C. Anthracite
- D. agarose and carbodiimide Discuss
Correct Answer: agarose and carbodiimide
- 4. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
Options- A. low molecular weight
- B. high molecular weight
- C. high equivalent weight
- D. low reactivity Discuss
Correct Answer: low molecular weight
- 5. The immobilized technique involving chemical method is
Options- A. covalent bond formation dependent
- B. non-covalent bond formation dependent
- C. both (a) and (b)
- D. ionic bond formation dependent Discuss
Correct Answer: covalent bond formation dependent
- 6. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
Options- A. equal to that of substrate consumption
- B. more than that of substrate consumption
- C. lesser than that of substrate consumption
- D. is nothing to do with the substrate consumption Discuss
Correct Answer: equal to that of substrate consumption
- 7. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options- A. free ?-, ?- or ? carb oxyl groups
- B. ? or ? amino groups
- C. phenyl, hydroxyl, sulfhydryl or imidazole groups
- D. all of the above Discuss
Correct Answer: all of the above
- 8. Which of the following is taken as an assumption in the distributed model?
Options- A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
- B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
- C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
- D. All of the above Discuss
Correct Answer: All of the above
- 9. The covalent attachment of enzyme molecules is via
Options- A. nonessential amino acids residues to water insoluble, functional supports
- B. essential amino acids residues to water insoluble, functional supports
- C. nonessential amino acids residues to water soluble, functional supports
- D. essential amino acids residues to water soluble, functional supports Discuss
Correct Answer: nonessential amino acids residues to water insoluble, functional supports
- 10. For glucose isomerization by immobilized enzyme, the reactor generally used is
Options- A. CSTR
- B. plug flow
- C. packed bed
- D. fluidized bed Discuss
Correct Answer: packed bed
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