Question 1

Halophilic microbiota of mined salt: Reports on the native microflora of mined salt typically indicate which approximate distribution among common groups?

Accepted Answer

Introduction / Context: Even high-salt environments harbor specialized microflora. Understanding the typical composition of microorganisms in mined salt matters for salted food preservation, brine preparation, and quality expectations for traditional curing operations. Given Data / Assumptions: Mined salt contains halotolerant Gram-positive cocci (formerly grouped as Micrococcus), coryneform bacteria, and spore-forming Bacillus spp. The question asks for approximate proportions as reported in classical surveys. Exact percentages may vary by mine and sampling method; the answer reflects a commonly cited distribution used in exam settings. Concept / Approach: The dominance of micrococci in salt reflects their halotolerance and survival on dry crystal surfaces. Coryneforms are also salt-tolerant skin/environmental bacteria, and Bacillus spores persist due to desiccation resistance. A typical textbook distribution lists roughly 70% micrococci, 20% coryneforms, and around 10% Bacillus among isolates. Step-by-Step Solution: Recall the classic composition breakdown used in food microbiology curricula. Recognize that micrococci dominate > 50% in mined salt surveys. Select the option that aligns with 70/20/10 proportions. Verification / Alternative check: Historical culture-based studies on salt crystals and brines yield high counts of micrococci/coryneforms, with smaller fractions of Bacillus due to spore survival; modern sequencing adds archaea but classical exam answers rely on bacterial groups. Why Other Options Are Wrong: Other splits underrepresent micrococci or misstate the Bacillus fraction compared with the commonly taught 70/20/10 pattern. Common Pitfalls: Assuming sterility of mined salt; while counts are low, contaminants seed brines and salted foods if hygiene lapses occur. Final Answer: 70% Micrococcus, 20% coryneforms, and 10% Bacillus.

Question 2

Industrial fermentation (baker's yeast): The manufacture of baker's yeast for leavening bread primarily involves which microorganism?

Accepted Answer

Introduction / Context: Baker's yeast is the cornerstone of bread making and many leavened baked goods. The organism used must produce carbon dioxide efficiently, be safe for food use, and withstand industrial propagation, harvesting, and storage. This question tests recognition of the correct species employed worldwide in bakeries and yeast factories. Given Data / Assumptions: The target product is baker's yeast used to leaven dough via CO2 production from sugars. Options include yeasts and non-yeast microbes, some of which are used in other foods. Focus is on the organism predominantly propagated at industrial scale for bread. Concept / Approach: Saccharomyces cerevisiae is the standard organism for baker's yeast. It rapidly metabolizes fermentable sugars (glucose, fructose, maltose) via glycolysis and fermentation, producing CO2 that expands dough. It also imparts desirable aroma compounds. Industrial strains are selected for high biomass yield in aerobic fed-batch culture and robust performance in dough. Step-by-Step Solution: Match the product (baker's yeast) with the conventional production organism. Note that S. cerevisiae is optimized for CO2 generation and baking functionality. Exclude organisms mainly used for yogurt starters (Streptococcus thermophilus) or spoilage/enzymes (Bacillus subtilis). Select Saccharomyces cerevisiae as the correct answer. Verification / Alternative check: Commercial yeast packages and technical datasheets list S. cerevisiae for instant, active dry, and compressed forms. Brewing and wine strains are also S. cerevisiae variants, underscoring its food safety and performance record. Why Other Options Are Wrong: Streptococcus thermophilus: Dairy starter bacterium for yogurt; not a leavening yeast. Bacillus subtilis: Produces enzymes; certain strains cause rope spoilage in bread. None of these: Incorrect because S. cerevisiae is correct. Candida utilis: Used for SCP/biomass and flavor enhancers, not standard baker's yeast. Common Pitfalls: Confusing general "yeast" with any fungus; only specific Saccharomyces strains give reliable baking performance and gas production. Final Answer: Saccharomyces cerevisiae is the organism used to manufacture baker's yeast.

Question 3

Baker's yeast propagation medium: The most commonly used medium for culture build-up and production of baker's yeast consists of which components?

Accepted Answer

Introduction / Context: Industrial production of baker's yeast uses large aerobic fermenters and economical substrates. Media design must support high cell yields, maintain vitality, and control by-products such as ethanol. Understanding the standard formulation is fundamental in food and industrial microbiology. Given Data / Assumptions: Feedstocks should be low-cost and rich in fermentable sugars. Trace minerals and salts are required for enzyme cofactors and osmotic balance. The question seeks the broad component list used worldwide. Concept / Approach: Cane or beet molasses provides sucrose, minerals, vitamins, and growth factors, making it the dominant carbon source. However, additional minerals (e.g., magnesium, phosphate) and salts (e.g., ammonium sulfate as nitrogen, phosphate buffers) are supplemented to optimize biomass yield and prevent nutrient limitations during fed-batch growth. Step-by-Step Solution: Identify molasses as the economical sugar source for yeast biomass. Recognize the need for mineral and salt supplementation for balanced growth. Reject options lacking supplements or substituting lactose (S. cerevisiae lacks efficient lactose use). Choose “Molasses, minerals, and salts.” Verification / Alternative check: Process manuals describe molasses with ammonium salts, phosphates, magnesium, and vitamins, plus antifoam and pH control, as the standard recipe for baker's yeast propagation. Why Other Options Are Wrong: Molasses only / Molasses and minerals: Incomplete without proper salts and nitrogen sources. Molasses and lactose: Lactose metabolism is poor in typical baker's yeast strains. Refined sucrose without minerals: More costly and nutritionally incomplete. Common Pitfalls: Assuming molasses composition alone suffices; variability in molasses requires precise mineral and nitrogen supplementation. Final Answer: Molasses, minerals, and salts form the common production medium.

Question 4

Soft drink spoilage (yeasts and bacteria): Which defect is a major result of marked microbial growth in carbonated soft drinks?

Accepted Answer

Introduction / Context: Soft drinks are high-sugar, often acidic beverages that should be microbiologically stable if processed correctly. When yeasts and certain bacteria proliferate, they create characteristic defects that compromise clarity, texture, and mouthfeel. Recognizing the hallmark defect helps direct quality control. Given Data / Assumptions: We focus on defects caused by yeast/bacterial growth in soft drinks (not canned low-acid foods). Products are typically clarified and filtered; turbidity indicates microbial proliferation. Some bacteria produce extracellular polymers that cause viscosity issues. Concept / Approach: Cloudiness arises when microbial biomass and by-products scatter light in otherwise clear beverages. Ropiness is due to exopolysaccharides produced by organisms such as certain lactic acid bacteria or Zymomonas, resulting in viscous, slimy strands. Yeasts (e.g., Saccharomyces, Candida) also cloud beverages and ferment sugars, producing CO2 and off-flavors. These are classic defects for sweet, acidic soft drinks with preservative failures or post-contamination. Step-by-Step Solution: List common microbial defects in soft drinks. Match yeasts/bacteria to turbidity and exopolysaccharide production. Identify “cloudiness and ropiness” as the primary combined manifestation. Select option reflecting both clarity loss and viscosity change. Verification / Alternative check: Quality manuals cite ropiness and turbidity as typical microbial spoilage signs in soft drinks, often with gas production and package swelling if yeasts grow. Why Other Options Are Wrong: Flat sour spoilage: Pertains to canned foods by thermophilic spore-formers, not soft drinks. Superficial fungal spoilage / Pin-spot molding: Surface molds occur more in juices or inadequately closed containers; not the principal yeast/bacterial soft drink defect. Glass corrosion: Chemical/packaging issue, not a microbial growth signature. Common Pitfalls: Confusing carbonation loss with “flat sour”; the latter is a specific canned food defect unrelated to beverage clarity. Final Answer: Cloudiness and ropiness are major spoilage outcomes of yeast/bacterial growth in soft drinks.

Question 5

Koji manufacture (starter for soy sauces and miso): The most important organism(s) involved in koji production is/are which of the following?

Accepted Answer

Introduction / Context: Koji is a mold-cultured grain (often rice or wheat) that serves as the enzyme-rich starter for Japanese fermentations such as soy sauce, miso, and sake. The mold must secrete powerful amylases and proteases to saccharify starches and hydrolyze proteins, preparing substrates for subsequent fermentations. Given Data / Assumptions: We identify the principal koji molds used traditionally and industrially. Organisms must be safe, high enzyme producers, and well-adapted to solid-state culture. Concept / Approach: Aspergillus oryzae and Aspergillus sojae are the two classic koji molds. They produce amylases, proteases, and peptidases essential for generating sugars, peptides, and amino acids that later yeasts and bacteria transform into flavor and aroma compounds. These Aspergillus strains are domesticated, with long histories of safe use. Step-by-Step Solution: List candidate organisms and their roles. Associate Aspergillus oryzae/sojae with koji enzyme production. Exclude lactic bacteria and other molds not central to koji. Choose the combined option “Both (a) and (b).” Verification / Alternative check: Traditional manuals and modern factories alike rely on A. oryzae/sojae for controlled koji growth and enzyme release at 28–35 °C with careful aeration and moisture control. Why Other Options Are Wrong: Lactococcus lactis: Dairy starter; not a koji mold. Rhizopus oligosporus: Used for tempeh fermentation of soybeans, not koji. Common Pitfalls: Confusing koji with tempeh or misidentifying koji as a yeast culture; koji is primarily a filamentous mold culture. Final Answer: Both (a) and (b) — Aspergillus oryzae and Aspergillus sojae — are the key koji molds.

Question 6

Stored-grain spoilage and mycotoxins: Which mold genera are commonly implicated in the spoilage of stored grains and associated health hazards?

Accepted Answer

Introduction / Context: Stored grains can support mold growth when moisture and temperature are not controlled. Beyond economic losses from spoilage, certain molds produce mycotoxins that pose serious animal and human health risks. Identifying the principal genera is critical for prevention and monitoring. Given Data / Assumptions: We consider common storage fungi and field fungi that persist into storage. Health hazards include aflatoxins, ochratoxin, fumonisins, trichothecenes, and zearalenone. Concept / Approach: Aspergillus (e.g., A. flavus, A. parasiticus) contaminates warm, humid grain, producing aflatoxins. Penicillium species can grow at lower temperatures and generate ochratoxin A and patulin (more in fruits). Fusarium species (field fungi) produce trichothecenes and fumonisins that may carry into storage. All three genera are relevant to grain spoilage and safety. Step-by-Step Solution: List major toxin-producing genera in grains: Aspergillus, Penicillium, Fusarium. Recognize overlapping ecological niches across harvest and storage phases. Select the inclusive option, "All of these." Verification / Alternative check: Grain standards worldwide test for aflatoxins and Fusarium toxins; storage guidelines target moisture below safe thresholds (e.g., water activity < 0.70–0.75) to inhibit these molds. Why Other Options Are Wrong: Single-genus options are incomplete. Rhizopus only: More common in high-moisture spoilage; not the primary toxin focus in stored grains. Common Pitfalls: Assuming cold storage eliminates risk; Penicillium species can grow at low temperatures if moisture is adequate. Final Answer: All of these genera are common in stored-grain spoilage and hazards.

Question 7

Idli fermentation microbiology: Which organism is present in the initial stages of natural idli batter fermentation (soaked rice and black gram)?

Accepted Answer

Introduction / Context: Idli is a traditional South Asian steamed cake made from a fermented batter of rice and dehulled black gram (urad). The microbial succession during soaking and grinding creates leavening, acidification, and flavor. Recognizing early colonizers explains gas formation and pH drop that set the stage for lactic acid bacteria. Given Data / Assumptions: Batter is fermented spontaneously from microorganisms on grains and equipment. Initial organisms drive early CO2 formation and slight acidity at ambient temperatures. Later stages are dominated by lactic acid bacteria that stabilize acidity and texture. Concept / Approach: Leuconostoc mesenteroides (a heterofermentative lactic bacterium) commonly appears early, producing CO2, lactic acid, and flavor compounds. This gas creates aeration in batter. As fermentation proceeds, Lactobacillus species (e.g., L. plantarum) increase acidification and contribute to texture and shelf-life. Molds like Aspergillus niger are not part of desired batter flora, and Bacillus natto pertains to soybean fermentation. Step-by-Step Solution: Identify early heterofermentative LAB in cereal-pulse fermentations. Connect CO2 production and mild acidity to Leuconostoc mesenteroides. Exclude molds and unrelated Bacillus fermentations. Select L. mesenteroides as the initial organism. Verification / Alternative check: Studies of idli batter show early dominance by Leuconostoc spp., followed by Lactobacillus spp.; gas bubble formation corresponds with heterofermentation. Why Other Options Are Wrong: Aspergillus niger: Environmental mold; not desired in idli batter. Bacillus natto: Soybean fermentation organism; not typical in idli. Lactobacillus plantarum: Important later but not the earliest dominant. None of these: Incorrect because L. mesenteroides is correct. Common Pitfalls: Attributing early batter aeration to yeasts alone; in idli, heterofermentative LAB play a key role in gas formation. Final Answer: Leuconostoc mesenteroides predominates in the initial stage of idli fermentation.

Question 8

Food microbiology — bakery spoilage: In commercial bread, the defect called “ropiness” (sticky, stringy crumb with fruity odour) is typically caused by which microorganism?

Accepted Answer

Introduction / Context: Ropiness in bread is a classic spoilage problem in baking and food microbiology. It presents as a sticky, stringy crumb and an unpleasant sweet or fruity odour, often noticed after slicing. Identifying the responsible organisms helps bakers select appropriate control steps such as sanitation, cooling practices, and the use of preservatives. Given Data / Assumptions: The question asks for the primary causative agent of rope spoilage in bread. Candidate organisms include bacteria and molds commonly encountered in bakery environments. Assume standard bread made from wheat flour, baked and then cooled/stored at ambient conditions. Concept / Approach: Rope spoilage is chiefly bacterial, not fungal. The main agents are Bacillus species (historically Bacillus subtilis and Bacillus licheniformis). Their spores survive baking and can germinate in warm, moist bread during slow cooling or improper storage. They produce extracellular enzymes that degrade starches and proteins, causing the characteristic sticky strands and off-odours. Step-by-Step Solution: Link the named defect “ropiness” specifically with Bacillus spp. (rope-forming bacteria). Recall that Bacillus spores can withstand baking and later germinate in the loaf. Recognize that common bakery molds (Aspergillus, Rhizopus, Penicillium) cause visible mold growth, not the rope phenotype. Select the organism classically associated with rope: Bacillus subtilis. Verification / Alternative check: Quality manuals note rope occurrence increases with warm weather, slow cooling, and high loaf moisture. Acidification (e.g., sourdough), use of approved preservatives (such as propionates), rapid cooling, and strict sanitation reduce the risk. Why Other Options Are Wrong: Aspergillus niger / Rhizopus nigricans / Penicillium expansum: These molds typically cause surface mold growth and visible colonies rather than rope; they are not the hallmark rope agents. Candida albicans: A yeast associated with clinical settings; not the classic bread rope organism. Common Pitfalls: Confusing any post-bake spoilage with mold growth; rope is a bacterial spoilage with a distinctive stringy crumb and fruity odour, not fuzzy mold. Final Answer: Bacillus subtilis is the typical cause of ropiness in bread.

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