During which fundamental process is an exact new copy of a DNA molecule synthesized?
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ATransformation (uptake of naked DNA)
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BTranscription (DNA-directed RNA synthesis)
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CTranslation (mRNA-directed protein synthesis)
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DReplication (DNA-directed DNA synthesis)
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ERecombination (exchange between DNA molecules)
Answer
Correct Answer: Replication (DNA-directed DNA synthesis)
Explanation
Introduction / Context:Central dogma steps—replication, transcription, and translation—have distinct information flows. Only one process duplicates the genetic material itself to pass to daughter cells: replication.
Given Data / Assumptions:
- The question asks about producing a precise, complementary copy of a DNA molecule.
- Other listed processes relate to RNA synthesis, protein synthesis, or DNA exchange.
Concept / Approach:Replication is semi-conservative: each daughter duplex contains one parental and one newly synthesized strand. DNA polymerases copy templates in the 5′→3′ direction using primers. Transcription produces RNA, translation produces polypeptides, transformation and recombination alter DNA content but do not copy the entire DNA molecule as a dedicated process.
Step-by-Step Solution:Define “exact new copy” as duplication of the DNA duplex.Identify the enzymatic process that accomplishes this: DNA replication.Exclude transcription (DNA→RNA), translation (RNA→protein), and transformation/recombination (DNA movement/reshuffling).
Verification / Alternative check:Meselson–Stahl experiments demonstrated semi-conservative replication using isotope labeling of DNA strands, directly confirming the mechanism.
Why Other Options Are Wrong:
- Transformation: introduces external DNA but does not copy the entire genome.
- Transcription: creates RNA, not DNA.
- Translation: uses mRNA to build proteins.
- Recombination: rearranges DNA segments, not wholesale duplication.
Common Pitfalls:Equating “trans” words (transcription/translation/transformation) with duplication; only replication duplicates DNA.
Final Answer:Replication (DNA-directed DNA synthesis)