MacConkey agar classification: what kind of medium is it? In clinical microbiology, MacConkey medium is best classified as which type of culture medium based on its functional properties?

Difficulty: Easy

Correct Answer: Differential medium

Explanation:


Introduction / Context:
MacConkey agar is ubiquitous in diagnostic bacteriology for isolating enteric organisms and distinguishing lactose fermenters from nonfermenters. Correctly categorizing the medium helps students predict which organisms will grow and how colonies will appear.


Given Data / Assumptions:

  • MacConkey contains bile salts and crystal violet (inhibit many Gram-positive organisms).
  • It includes lactose and a pH indicator that differentiates fermentation.
  • Transport media are designed to preserve viability during transit, not to differentiate.


Concept / Approach:

While MacConkey is indeed both selective (for Gram-negative enterics) and differential (lactose fermentation), many question banks emphasize its hallmark utility: differentiating lactose fermenters (pink/red colonies) versus nonfermenters (colorless). Given single-answer format, “differential medium” is typically the expected response; “all of these” is incorrect because MacConkey is not a transport medium or simple enrichment medium.


Step-by-Step Solution:

Identify core function: lactose fermentation indicator distinguishes colony types.Note selectivity is present, but transport/enrichment functions are not.Choose “Differential medium” as the best single classification.


Verification / Alternative check:

Laboratory manuals describe MacConkey as selective and differential; in single-answer questions, “differential medium” is often keyed because the visible outcome is differential colony color.


Why Other Options Are Wrong:

  • Transport medium: MacConkey is not used for sample transport.
  • Enrichment medium: It does not broadly enrich; it selects and differentiates.
  • All of these: False because transport/enrichment do not apply.
  • Selective and differential medium: Factually true, but the question uses mutually exclusive categories with one correct key; many curricula expect “differential”.


Common Pitfalls:

  • Assuming “all of these” whenever more than one property applies; always verify each term.


Final Answer:

Differential medium

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