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  • Question
  • A fed-batch reactor initially contains 2 litre of medium. If it was fed at 1 litre per hour, then after 10 hours, the volume of the reactor will be


  • Options
  • A. 1 litre
  • B. 2 litre
  • C. 3 litre
  • D. 13 litre

  • Correct Answer
  • 2 litre 


  • Fermentation Reactors problems


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    • 1. If biomass yields are constant, then the biomass productivity of a culture grown in continuous reactor will

    • Options
    • A. always decrease with dilution rate
    • B. increase with dilution rate until washout
    • C. remain constant irrespective of the dilution rate
    • D. decrease with dilution rate until washout
    • Discuss
    • 2. A continuous bioreactor in which only the flow rate is used to control the rate of cell or product productivity is called

    • Options
    • A. turbidostat
    • B. chemostat
    • C. level stat
    • D. pH
    • Discuss
    • 3. Yield coefficient represents

    • Options
    • A. total biomass or product produced
    • B. conversion efficiency of a substrate into product
    • C. conversion rate of a substrate into biomass or product
    • D. production time of biomass or product
    • Discuss
    • 4. Formation of end product by Lactococcus lactis will become non-growth associated as lactic acid accumulates because

    • Options
    • A. cells will redirect ATP to anabolism
    • B. cells will redirect NAD+ to anabolism
    • C. cells will redirect ATP to facilitate the diffusion of lactic acid and H+ out of the cells
    • D. cells will redirect ATP to the active transport of lactic acid and H+ out of the cells
    • Discuss
    • 5. A continuous reactor has a dilution rate of 0.5 h-1. Its residence time would be

    • Options
    • A. ln(2)/0.5
    • B. ln(2) x 0.5
    • C. 0.5 h
    • D. 2 h
    • Discuss
    • 6. When two populations compete for a single growth limiting substrate in a continuous fermenter, which organism would not be washed out?

    • Options
    • A. Organism maintaining the highest substrate concentration
    • B. Organism maintaining the lowest substrate concentration
    • C. Both (a) and (b)
    • D. Organism maintaining the moderate substrate concentration
    • Discuss
    • 7. In fed-batch fermentation, mass of substrate utilized is calculated using the relationship

    • Options
    • A. initial mass of substrate in the reactor + final mass of substrate in the reactor + flow rate x [substrate in the feed]
    • B. initial mass of substrate in the reactor - final mass of substrate in the reactor + flow rate x [substrate in the feed]
    • C. initial mass of substrate in the reactor + final mass of substrate in the reactor - flow rate x [substrate in the feed]
    • D. initial mass of substrate in the reactor + final mass of substrate in the reactor - flow rate
    • Discuss
    • 8. Saccharomyces cerevisiae is being grown in a chemostat converts glucose to biomass, ethanol, glycerol and carbon dioxide. At steady state, the concentration of glucose, biomass, ethanol and glycerol will

    • Options
    • A. decrease with time
    • B. increase with time
    • C. be constant
    • D. change randomly with time
    • Discuss
    • 9. Which of the statement is true for continuous reactor at steady state?

    • Options
    • A. The rates of biomass, substrate and product concentrations are zero
    • B. Biomass, substrate and product concentrations are zero
    • C. Biomass, substrate and product concentrations do not change with time
    • D. Biomass, substrate and product concentrations change with time
    • Discuss
    • 10. Which of the following is used to calculate mass of substrate in the reactor?

    • Options
    • A. Flow rate x substrate concentration in the reactor
    • B. Volume of reactor x substrate concentration in reactor
    • C. Flow rate x mass of reactor
    • D. Volume of reactor x Flow rate
    • Discuss


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