Routine monitoring of the microbial quality of treated piped drinking water supplies is primarily carried out using which test?

Difficulty: Easy

Correct Answer: Coliform Most Probable Number (MPN) test

Explanation:


Introduction / Context:
Public health surveillance of water supplies relies on indicator organisms that signal fecal contamination and treatment efficacy. Direct routine testing for every pathogen is impractical; instead, coliform bacteria (particularly E. coli) serve as reliable indicators of sanitary quality in distribution systems.


Given Data / Assumptions:

  • Treated, piped drinking water supply under routine monitoring.
  • Indicator approach preferred over exhaustive pathogen identification.
  • Laboratory capacity typical of municipal water quality programs.


Concept / Approach:
The Coliform MPN test estimates the most probable number of coliform organisms per 100 mL using serial dilution and fermentation tubes or membrane filtration counts. The presence/absence and counts of coliforms correlate with the likelihood of fecal contamination and disinfection failures. Heterotrophic plate counts are supplementary process indicators but do not directly indicate fecal pollution.


Step-by-Step Solution:

Collect representative distribution system samples aseptically.Perform MPN or membrane filtration for total coliforms and E. coli as per standards.Interpret results against regulatory criteria (e.g., zero E. coli in 100 mL).


Verification / Alternative check:

Cross-check with chlorine residuals and turbidity; spikes in HPC may prompt operational reviews but are not fecal indicators per se.


Why Other Options Are Wrong:

Microscopy and heterotrophic plate counts lack specificity to fecal contamination.Identifying all pathogens or universal viral assays are infeasible for routine monitoring.


Common Pitfalls:

Relying solely on HPC; ignoring sample preservation and holding times that can alter bacterial counts.


Final Answer:

Coliform Most Probable Number (MPN) test

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