Difficulty: Easy
Correct Answer: burst size
Explanation:
Introduction / Context:
In one-step growth experiments with bacteriophages, key time points and quantities describe viral replication dynamics. Understanding these terms helps interpret growth curves and infection kinetics. This item asks for the term describing the number of phage progeny produced per infected host cell.
Given Data / Assumptions:
Concept / Approach:
The burst size is defined as the average number of mature phage particles released from one bacterium at lysis. It depends on phage type, host physiology, and conditions. The eclipse period is when no mature intracellular phage are detectable; the latent period spans from adsorption to the first release of mature phage; the rise period is when extracellular titer increases to a plateau.
Step-by-Step Solution:
Verification / Alternative check:
Perform multiplicity-of-infection–controlled assays and compare counts pre- and post-lysis to derive the same number.
Why Other Options Are Wrong:
Common Pitfalls:
Confusing time intervals (eclipse/latent) with quantitative yield; ignoring partial infections or superinfection effects.
Final Answer:
burst size
Discussion & Comments