Enzymology — “Taka-diastase,” an industrial amylolytic enzyme preparation used to hydrolyze starch, is produced by which microorganism?
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AA. oryzae
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BB. subtilis
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CA. niger
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DS. cerevisiae
Answer
Correct Answer: A. oryzae
Explanation
Introduction / Context:Taka-diastase is a classic preparation rich in amylases used for starch liquefaction and saccharification. Recognizing its microbial origin is a staple question in industrial microbiology and food biotechnology.
Given Data / Assumptions:
- The name “taka-diastase” historically refers to enzyme extracts from koji molds.
- Aspergillus species are leading industrial enzyme producers.
- We must identify the specific organism associated with taka-diastase.
Concept / Approach:Aspergillus oryzae (the koji mold) is famed for producing strong amylases used in sake, soy sauce, and enzyme industries. While A. niger also produces amylases, “taka-diastase” traditionally refers to A. oryzae products.
Step-by-Step Solution:
Connect taka-diastase to koji fermentations.Identify the koji mold: Aspergillus oryzae.Select A. oryzae as the correct answer.Verification / Alternative check:Historic enzyme manufacturing texts and food fermentation literature consistently tie taka-diastase to A. oryzae.
Why Other Options Are Wrong:
- B. subtilis: industrial proteases, not the namesake of taka-diastase.
- A. niger: amylases yes, but taka-diastase specifically credits A. oryzae.
- S. cerevisiae: a yeast; not the amylase source in this context.
Common Pitfalls:Assuming any amylase-producing mold equals taka-diastase; terminology is historically specific.
Final Answer:A. oryzae