Amino acid classification by polarity and charge: Which of the following trios is NOT a sensible grouping based on side-chain polarity/charge properties used in biochemistry courses and protein science?

Introduction / Context:
Amino acids are commonly grouped by their side-chain properties because polarity and charge strongly influence protein folding, enzyme catalysis, and membrane interactions. Typical sets include nonpolar aliphatic (e.g., Ala, Val, Leu), polar/uncharged (e.g., Ser, Thr, Asn, Gln), aromatic (e.g., Phe, Tyr, Trp), acidic (Asp, Glu), and basic (Lys, Arg, His).

Given Data / Assumptions:

• We are judging whether each trio represents a coherent polarity/charge class.
• Standard undergraduate/graduate biochemistry conventions apply.
• Minor nuances (e.g., Tyr being more polar than Phe) are acknowledged but still fit the aromatic set.

Concept / Approach:
Cohesive groupings share similar polarity/charge: nonpolar aliphatics cluster together; basic residues cluster together; aromatics cluster together; mixing acidic with nonpolar residues is not sensible for polarity grouping.

Step-by-Step Solution:
Evaluate Ala, Leu, Val → all nonpolar, aliphatic: sensible.Evaluate Arg, His, Lys → basic/positively charged at physiological pH range: sensible (His is weakly basic but grouped here).Evaluate Phe, Trp, Tyr → aromatic set: sensible despite Tyr’s phenolic OH increasing polarity.Evaluate Asp, Ile, Pro → Asp is acidic/polar negative; Ile is strongly hydrophobic; Pro is nonpolar cyclic imino acid: mixed properties → not a sensible polarity group.

Verification / Alternative check:
Most textbooks and protein parameter tables organize residue properties similarly; hydropathy scales support that Ile/Pro are hydrophobic while Asp is hydrophilic and negatively charged.

Why Other Options Are Wrong:

• Ala, Leu, Val: classic hydrophobic cluster.
• Arg, His, Lys: basic side chains, engage in salt bridges.
• Phe, Trp, Tyr: aromatic ring systems often grouped together.

Common Pitfalls:
Overthinking Tyr’s polarity and assuming it disqualifies the aromatic grouping; forgetting His’s context-dependent charge yet its placement among basics for pedagogy.

Final Answer:
Asp, Ile, and Pro