Animal tissue culture methods: which one of the following is NOT a classic “explantation” technique, but rather a general culture mode?

Introduction / Context:
Explantation techniques are historical and classic methods for cultivating small pieces of tissue or organ fragments (explant cultures). They include formats like Carrel flasks, roller tubes, and similar setups designed specifically for maintaining tissue fragments.

Given Data / Assumptions:

• “Explantation” emphasizes small tissue fragments maintained on or within a support (clots, gels, or surfaces).
• Carrel flask and roller tube systems are canonical explant/organ culture approaches.
• “Adherent primary culture” describes a general growth mode, not a specific explantation technique.

Concept / Approach:
We must distinguish named explant methods from broader descriptors. While slide culture can be used for certain tissue or microbial studies, and Carrel/roller formats are classic explant systems, “adherent primary culture” simply indicates cells growing attached to a surface—many culture types can be adherent and primary without being a named explant technique.

Step-by-Step Solution:

Verification / Alternative check:
Standard references classify Carrel/roller/hanging-drop/plasma clot/raft as explant methods, whereas adherent/ suspension are general modes.

Why Other Options Are Wrong:

• A/B/C/E: Each corresponds to a recognized explant culture setup or format.

Common Pitfalls:
Confusing “explant culture” (tissue pieces) with “primary culture” (cellular outgrowth), and using growth mode labels in place of technique names.

Final Answer:
Adherent primary culture