Difficulty: Easy
Correct Answer: All of these
Explanation:
Introduction:
Imaging technique determines the level of cellular detail you can resolve. This question tests conceptual understanding of why electron microscopy (EM) outperforms light microscopy (LM) across diverse cell types by achieving much higher resolution and magnification.
Given Data / Assumptions:
Concept / Approach:
Resolution is the minimum distance at which two points are distinguishable. LM is limited by visible light wavelengths and numerical aperture, while EM uses electron beams with far shorter effective wavelengths, yielding markedly better resolution.
Step-by-Step Solution:
Verification / Alternative check:
Typical LM resolution is on the order of ~200 nm, whereas transmission EM can reach sub-nanometer scales in favorable conditions, explaining consistent superiority in visible detail across cells.
Why Other Options Are Wrong:
Common Pitfalls:
Confusing contrast with resolution; staining can improve LM contrast, but not surpass the diffraction limit. EM requires vacuum and fixed specimens, but this does not negate its superior resolving power.
Final Answer:
All of these.
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