Which bacterial colonies show brick-red fluorescence under ultraviolet (UV) light? (Consider classic oral black-pigmented anaerobes.)
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AB. gingivalis
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BB. melaninogenicus
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CBacteroides fragilis
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DB. levii
Answer
Correct Answer: B. melaninogenicus
Explanation
Introduction: Some black-pigmented oral anaerobes demonstrate characteristic fluorescence under UV light, aiding presumptive identification. This question asks for the species known for brick-red fluorescence.
Given Data / Assumptions:
- Nomenclature reflects historical names common in question banks.
- UV fluorescence is observed on blood agar under anaerobic culture.
Concept / Approach: “B. melaninogenicus” (now Prevotella melaninogenica) is classically described as exhibiting brick-red fluorescence. Porphyromonas gingivalis is typically asaccharolytic and pigmented but less consistently brick-red fluorescent in standard descriptions.
Step-by-Step Solution: Recall which black-pigmented anaerobes fluoresce in UV light. Match the canonical example of brick-red fluorescence to B. melaninogenicus. Exclude non-fluorescent or inconsistently fluorescent species.
Verification / Alternative check: Classic lab manuals list P. melaninogenica among species with brick-red fluorescence, supporting its use as a teaching example.
Why Other Options Are Wrong:
- B. gingivalis: Pigmented but not the standard brick-red fluorescent exemplar.
- Bacteroides fragilis: Non-pigmented gut anaerobe; does not show brick-red fluorescence.
- B. levii: Less commonly cited for this specific fluorescence pattern.
Common Pitfalls: Assuming all black-pigmented anaerobes behave identically under UV; fluorescence patterns differ by species.
Final Answer: B. melaninogenicus shows brick-red fluorescence under UV light.