Which bacterial colonies show brick-red fluorescence under ultraviolet (UV) light? (Consider classic oral black-pigmented anaerobes.)

Difficulty: Easy

Correct Answer: B. melaninogenicus

Explanation:


Introduction:
Some black-pigmented oral anaerobes demonstrate characteristic fluorescence under UV light, aiding presumptive identification. This question asks for the species known for brick-red fluorescence.


Given Data / Assumptions:

  • Nomenclature reflects historical names common in question banks.
  • UV fluorescence is observed on blood agar under anaerobic culture.


Concept / Approach:
“B. melaninogenicus” (now Prevotella melaninogenica) is classically described as exhibiting brick-red fluorescence. Porphyromonas gingivalis is typically asaccharolytic and pigmented but less consistently brick-red fluorescent in standard descriptions.


Step-by-Step Solution:
Recall which black-pigmented anaerobes fluoresce in UV light. Match the canonical example of brick-red fluorescence to B. melaninogenicus. Exclude non-fluorescent or inconsistently fluorescent species.


Verification / Alternative check:
Classic lab manuals list P. melaninogenica among species with brick-red fluorescence, supporting its use as a teaching example.


Why Other Options Are Wrong:

  • B. gingivalis: Pigmented but not the standard brick-red fluorescent exemplar.
  • Bacteroides fragilis: Non-pigmented gut anaerobe; does not show brick-red fluorescence.
  • B. levii: Less commonly cited for this specific fluorescence pattern.


Common Pitfalls:
Assuming all black-pigmented anaerobes behave identically under UV; fluorescence patterns differ by species.


Final Answer:
B. melaninogenicus shows brick-red fluorescence under UV light.

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