Recombinant DNA Technology Questions

Gene function — Which listed gene encodes a protein central to white blood cell (neutrophil) microbicidal activity?

Tumor suppressors — The TP53 gene on chromosome 17 encodes a protein with which primary cellular role?

Molecular markers — What best defines a restriction fragment length polymorphism (RFLP) in genomic DNA?

Molecular cloning — What core features define an expression vector used to express a cloned gene in a host cell (consider replication, selection, and transcriptional control)?

Genetic mapping basics — Which statement is correct with respect to locating genetic traits using molecular tools such as restriction enzymes and markers?

Plant genetic transformation — Which method can directly deliver DNA into plant cells to obtain stable transformants?

Restriction cloning — To insert a foreign gene into a plasmid vector so that the ends are compatible, what must be true for both DNA molecules?

Gel electrophoresis of DNA — Because DNA is negatively charged, it migrates through an agarose/polyacrylamide gel from which electrode toward which electrode?

Cancer genetics — Which blotting technique is most directly used to detect DNA-level changes that can identify a defective gene implicated in cancer predisposition or development?

Restriction enzymes — What do isoschizomers recognize when comparing different restriction endonucleases?

Gene therapy feasibility — Which of the following genetic diseases is most amenable to correction by gene engineering because it is caused by a single defective gene?

History of restriction enzymes — Which was the first Type II restriction endonuclease to be discovered and characterized?

Allele-specific detection — Some genetic diseases cannot be diagnosed by changes in restriction sites. What are allele-specific oligonucleotide (ASO) probes used for in such cases?

Prenatal Diagnosis—Applications of Amniocentesis Which of the following genetic or chromosomal conditions can be diagnosed prenatally using amniocentesis, where fetal cells and amniotic fluid are analyzed by karyotyping and/or molecular tests?

Biosafety—Horizontal Gene Transfer Risk The transfer of antibiotic-resistance genes from genetically engineered bacteria to pathogenic bacteria in natural settings __________.

Restriction Enzymes—Naming Convention Restriction endonucleases are named according to which primary criterion?

Sticky-End Compatibility—Choosing Enzyme Pairs Which of the following pairs of restriction endonucleases will generate compatible (complementary) cohesive ends that can be ligated together in cloning?

Gene Function—Proteins Implicated in Growth Which of these human genes encodes a protein that directly plays a role in growth regulation?

Transgenic Crops—Golden Rice Phenotype Why does Golden Rice appear pale yellow in color when compared with conventional white rice?

Restriction Enzyme Classes—ATP Requirement Which class of restriction endonucleases typically does not require ATP hydrolysis for DNA cleavage under standard reaction conditions?