Immobilized Enzyme problems
- 1. The effectiveness factor increases with the
Options- A. increase of diffusivity and decreased with the increase of particle size
- B. increase of diffusivity and increased with the increase of particle size
- C. decrease of diffusivity and decreased with the increase of particle size
- D. increase of diffusivity and decrease with the decrease of particle size Discuss
Correct Answer: increase of diffusivity and decreased with the increase of particle size
- 2. The most commonly employed cross-linked polymer is the
Options- A. polyacrylamide gel
- B. collagen
- C. celluloses
- D. cation exchange resin Discuss
Correct Answer: polyacrylamide gel
- 3. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options- A. It is possible to separate and purify the enzymes while being immobilized
- B. The enzymes are not usually deactivated by adsorption
- C. The adsorption is a reversible process
- D. State of immobilization is very sensitive to solution pH, ionic strength and temperature Discuss
Correct Answer: State of immobilization is very sensitive to solution pH, ionic strength and temperature
- 4. The immobilized enzyme produced by micro encapsulation technique provides
Options- A. an extremely large surface area
- B. smaller surface area
- C. high amount of solvent
- D. relatively smaller surface area Discuss
Correct Answer: an extremely large surface area
- 5. Which of the following is the commonly employed adsorbents?
Options- A. Calcium carbonate
- B. Alumina
- C. Celluloses
- D. All of these Discuss
Correct Answer: All of these
- 6. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
Options- A. a diffusion cell with two compartments divided by a thin gel
- B. a diffusion cell with two compartments
- C. a diffusion cell with two compartments plus by a thin gel
- D. a diffusion cell with two compartments multiply by a thin gel Discuss
Correct Answer: a diffusion cell with two compartments divided by a thin gel
- 7. Which medium is used for the production of Penicillin using immobilized cells
Options- A. 1% peptone medium
- B. glucose medium
- C. Yeast extract medium
- D. LB broth Discuss
Correct Answer: glucose medium
- 8. Which of the following is not a physical method of immobilization?
Options- A. Adsorption
- B. Entrapment
- C. Micro encapsulation
- D. None of these Discuss
Correct Answer: None of these
- 9. Damkohler number(NDa) is
Options- A. the ratio of the maximum reaction rate to the maximum mass transfer rate
- B. the ratio of the minimum reaction rate to the maximum mass transfer rate
- C. the ratio of the maximum reaction rate to the minimum mass transfer rate
- D. the ratio of the minimum reaction rate to the minimum mass transfer rate Discuss
Correct Answer: the ratio of the maximum reaction rate to the maximum mass transfer rate
- 10. If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
Options- A. rp = ksa(Csb-Cs)
- B. rp = ksaCsb
- C. rp = ksaCs
- D. rp = ksa(Csb+Cs) Discuss
Correct Answer: rp = ksaCsb
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