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Enzymes and Kinetics problems

  • 1. Most enzymes work by

  • Options
  • A. increasing energy of activation
  • B. decreasing energy of activation
  • C. not affecting energy of activation
  • D. none of the above
    • Discuss
  • 2. When [S] = 0.1 *KM, the velocity of an enzyme catalyzed reaction is about:

  • Options
  • A. 0.1 * Vmax
  • B. 0.3 * Vmax
  • C. 0.5 * Vmax
  • D. 0.7 * Vmax
    • Discuss
  • 3. Which of the following refers to pseudo steady state?

  • Options
  • A. d(CE)/dt = 0
  • B. d(Cp)/dt = 0
  • C. d(CES)/dt = 0
  • D. d(Cs)/dt = d(CES)/dt
    • Discuss
  • 4. In the steady state the material balance equation for any component of a system is

  • Options
  • A. rate of addition + rate of removal - rate of formation = 0
  • B. rate of addition - rate of removal + rate of formation = 0
  • C. rate of addition + rate of removal + rate of formation = 0
  • D. none of the above
    • Discuss
  • 5. The equation for the rate of product formation for simple enzyme reaction is given by (Where rmax, maximum reaction rate, Cs substrate concentration, Cp product concentration ES, CES enzyme-substrate concentration)

  • Options
  • A. rp = rmax Cs/(Km+Cs)
  • B. rp= rmax CES/(Km+ CES)
  • C. rp = rmax Cs/(Km+CES)
  • D. rp = rmax Cs/(Km+Cp)
    • Discuss
  • 6. Which of the following statement(s) regarding enzymes, is/are false?

  • Options
  • A. Enzymes are always proteins that function as catalysts
  • B. Enzymes provide activation energy for reactions
  • C. Enzyme activity can be regulated
  • D. Enzymes may be used many times for a specific reaction
    • Discuss
  • 7. The slope of Lineweaver Burk plot for Michaelis Menten equation is

  • Options
  • A. Vmax/Km
  • B. Km/Vmax
  • C. 1/Km
  • D. Km.Vmax
    • Discuss
  • 8. An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?

  • Options
  • A. 13.54? moles liter-1min-1
  • B. 6.68? moles liter-1min-1
  • C. 7.57? moles liter-1min-1
  • D. 17.8? moles liter-1min-1
    • Discuss
  • 9. For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of Vmax) observed at [S] = 2 KM will be

  • Options
  • A. 0.09
  • B. 0.33
  • C. 0.66
  • D. 0.91
    • Discuss
  • 10. The usual method(s) to solve rate equation of simple enzyme kinetics is/are

  • Options
  • A. Michaelis Menten approach
  • B. Briggs-Haldane approach
  • C. Numerical solution approach
  • D. all of these
    • Discuss

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