Enzymes and Kinetics Questions

• The rate-determining step of Michaelis Menten kinetics is
• The active site of an enzyme remains
• In a Lineweaver-Burk Plot, competitive inhibitor shows which of the following effect?
• The enzyme inhibition can occur by
• Which graphical method is used to determine an enzyme degree of cooperativity?
• Which of these enzymes contains a Zinc (Zn) ion?
• Non-competitive inhibitor of an enzyme catalyzed reaction
• The effect of non-competitive inhibition on a Lineweaver-Burk Plot is that
• Which category of enzymes belongs to class two in the international classification?
• Given an enzyme with a Km = 10m M and Vmax = 100 m mol/min. If [S] = 100 m M, which of the following will be true?
• Most enzymes work by
• When [S] = 0.1 *KM, the velocity of an enzyme catalyzed reaction is about:
• Which of the following refers to pseudo steady state?
• In the steady state the material balance equation for any component of a system is
• The equation for the rate of product formation for simple enzyme reaction is given by (Where rmax, maximum reaction rate, Cs substrate concentration, Cp product concentration ES, CES enzyme-substrate concentration)
• Which of the following statement(s) regarding enzymes, is/are false?
• The slope of Lineweaver Burk plot for Michaelis Menten equation is
• An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?
• For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of Vmax) observed at [S] = 2 KM will be
• The usual method(s) to solve rate equation of simple enzyme kinetics is/are