Temperate phage life cycle: In a lysogenized bacterial culture, the phage reproductive cycle is triggered by a process known as what?

Difficulty: Easy

Correct Answer: Induction

Explanation:


Introduction / Context:
Temperate bacteriophages can integrate into the host genome as prophage and remain quiescent under the control of a phage-encoded repressor. Under stress, the prophage can be activated to enter the lytic cycle. This activation step is called induction and is central to understanding lysogeny–lysis switches.


Given Data / Assumptions:

  • Lysogeny: prophage maintained in the host chromosome.
  • DNA-damaging conditions (e.g., UV) can inactivate repressors.
  • Once repression is relieved, lytic genes are expressed.


Concept / Approach:
In models such as phage lambda, the CI repressor maintains latency. SOS responses (RecA-mediated) cleave the repressor, resulting in induction. The prophage excises, replicates, and packages its DNA, culminating in host lysis and virion release.


Step-by-Step Solution:
Define lysogeny vs. lytic growth. Identify the control protein (repressor) that prevents lytic gene expression. Recognize environmental triggers that disable repression. Name the switch event: induction.


Verification / Alternative check:
Classic experiments show UV exposure of lysogens yields phage production after prophage excision, consistent with induction.


Why Other Options Are Wrong:
Infection precedes lysogeny; integration is the establishment step; repression maintains dormancy; packaging is a late lytic event, not the trigger.


Common Pitfalls:
Confusing “induction” with initial infection; the term specifically refers to reactivation of a prophage.


Final Answer:
Induction.

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