Difficulty: Easy
Correct Answer: coagulase activity
Explanation:
Introduction / Context:
Baird–Parker agar is a selective–differential medium commonly used to isolate presumptive Staphylococcus aureus from foods. It contains tellurite and egg yolk; S. aureus typically forms black, shiny colonies due to tellurite reduction, often with clear zones from lipolytic/lecithinase activity. However, laboratory identification requires a confirmatory test associated with this species: the coagulase test.
Given Data / Assumptions:
Concept / Approach:
Most clinically and food-borne significant S. aureus strains are coagulase-positive, differentiating them from many coagulase-negative staphylococci. While catalase is positive in all staphylococci (and thus non-discriminatory), and protease activity is not a standard confirmatory trait, coagulase is the classical confirmatory test paired with Baird–Parker isolation.
Step-by-Step Solution:
Verification / Alternative check:
Food microbiology standards specify coagulase testing (and often latex agglutination for clumping factor/protein A) to confirm S. aureus from Baird–Parker plates.
Why Other Options Are Wrong:
Common Pitfalls:
Assuming colony appearance alone is definitive; always run coagulase or equivalent confirmatory tests.
Final Answer:
coagulase activity
Discussion & Comments