Food microbiology — Colonies of <em>Staphylococcus aureus</em> grown on Baird–Parker agar are typically associated with which confirmatory property?

Difficulty: Easy

Correct Answer: coagulase activity

Explanation:


Introduction / Context:
Baird–Parker agar is a selective–differential medium commonly used to isolate presumptive Staphylococcus aureus from foods. It contains tellurite and egg yolk; S. aureus typically forms black, shiny colonies due to tellurite reduction, often with clear zones from lipolytic/lecithinase activity. However, laboratory identification requires a confirmatory test associated with this species: the coagulase test.


Given Data / Assumptions:

  • We are dealing with “suspected” colonies on Baird–Parker medium.
  • Final confirmation relies on biochemical properties characteristic of S. aureus.


Concept / Approach:
Most clinically and food-borne significant S. aureus strains are coagulase-positive, differentiating them from many coagulase-negative staphylococci. While catalase is positive in all staphylococci (and thus non-discriminatory), and protease activity is not a standard confirmatory trait, coagulase is the classical confirmatory test paired with Baird–Parker isolation.


Step-by-Step Solution:

Use Baird–Parker for selective isolation (appearance: black colonies, clear halo).Apply tube or slide coagulase test to suspected colonies.Confirm identity: coagulase positivity supports S. aureus.


Verification / Alternative check:
Food microbiology standards specify coagulase testing (and often latex agglutination for clumping factor/protein A) to confirm S. aureus from Baird–Parker plates.


Why Other Options Are Wrong:

  • Protease activity: not a routine confirmatory hallmark for S. aureus.
  • Catalase activity: shared by nearly all staphylococci; not specific.
  • None of these: incorrect; coagulase is the classical confirmatory marker.


Common Pitfalls:
Assuming colony appearance alone is definitive; always run coagulase or equivalent confirmatory tests.


Final Answer:
coagulase activity

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