In lactic acid fermentations neutralized with calcium carbonate, why is a high initial sugar concentration avoided in the medium?
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ACalcium lactate is not produced
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BCalcium lactate may crystallize from the broth and slow fermentation
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CExcess sugar itself crystallizes rapidly
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DNone of the above
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EHigh sugar eliminates catabolite repression
Answer
Correct Answer: Calcium lactate may crystallize from the broth and slow fermentation
Explanation
Introduction / Context: During industrial lactic acid production, neutralization is often done with calcium carbonate to maintain pH and to avoid acid inhibition. Process performance depends on sugar levels, by-product solubility, and mass transfer in viscous broths.
Given Data / Assumptions:
- Calcium lactate is the neutralized product formed in situ.
- High sugar concentrations raise ionic strength and solids, altering solubility.
- We aim to identify the main operational reason to avoid high sugar.
Concept / Approach: At elevated concentrations, calcium lactate can crystallize, increasing slurry viscosity and impeding mixing, aeration (if used), and heat removal. Crystallization can also trap cells/nutrients, slowing metabolic rates and complicating downstream processing.
Step-by-Step Solution: Assess option a: incorrect—calcium lactate is indeed produced under neutralization. Consider option b: correct—excessive calcium lactate crystallization hampers fermentation kinetics. Option c: sugar crystallization is not the central issue under normal fermentation temperatures. Choose option b as the practical process limitation.
Verification / Alternative check: Process descriptions recommend fed-batch sugar addition to keep concentrations moderate, precisely to avoid salt precipitation and viscosity spikes.
Why Other Options Are Wrong: They misstate chemistry or emphasize phenomena not primary to performance loss.
Common Pitfalls: Assuming “more sugar, faster fermentation”; osmotic stress and salt precipitation can slow or stall cultures.
Final Answer: Calcium lactate may crystallize from the broth and slow fermentation.