Recombinant DNA vocabulary: In genetic engineering, what does the term “chimera” most commonly refer to in the context of DNA constructs?

Introduction / Context:
The term “chimera” in recombinant DNA technology refers to a DNA molecule composed of sequences from different origins joined together. In practice, this often means a plasmid vector into which a foreign DNA fragment has been inserted, yielding a recombinant (chimeric) construct used for cloning or expression.

Given Data / Assumptions:

• Vector backbones carry ori and selectable markers.
• Foreign inserts can be cDNAs, genomic fragments, or synthetic genes.
• Joining is accomplished by ligation or recombination systems.

Concept / Approach:
Match the definition of a chimera to recombinant DNA: a single molecule comprising parts from different sources. Although “chimera” can also describe organisms with mixed cell lineages, in cloning it commonly denotes plasmids containing heterologous inserts created for propagation or expression in host cells.

Step-by-Step Solution:

Verification / Alternative check:
Sequencing across the junctions confirms that sequences from different origins have been covalently joined, satisfying the definition of a chimeric (recombinant) plasmid.

Why Other Options Are Wrong:

• Enzymes and viruses are tools or vectors but are not themselves chimeric constructs by definition here.
• Fungi are organisms, not DNA constructs.
• Synthetic amino acids are unrelated to the definition of a recombinant DNA chimera.

Common Pitfalls:
Confusing organismal chimeras (mosaic animals) with molecular chimeras (recombinant plasmids). Context determines the intended meaning in genetics discussions.

Final Answer:
A plasmid that contains foreign DNA (a recombinant DNA molecule)