Plant biotechnology prerequisite — why embryogenic cell suspensions matter In plant tissue culture and genetic engineering, the establishment of embryogenic cell suspensions with high regeneration potential is a critical prerequisite for successful downstream work. This prerequisite is especially essential for which technique or application?

Introduction / Context:
Many plant biotechnology pipelines require regenerating complete plants from single cells. Embryogenic cell suspensions provide a uniform, highly regenerative starting material that reliably forms somatic embryos or shoots. This capability is indispensable for techniques that transiently or stably alter single cells and then need whole-plant recovery.

Given Data / Assumptions:

• The question focuses on plant systems that need high regeneration capacity.
• Embryogenic suspensions consist of totipotent cells capable of forming embryos.
• Success is defined by recovery of fertile plants, not merely molecular analysis.

Concept / Approach:

Protoplast culture involves enzymatically removing the cell wall, manipulating isolated cells (fusion, DNA uptake), and then regenerating the wall, callus, shoots, and eventually whole plants. This workflow fails without vigorous morphogenic competence. Embryogenic suspensions maximize the probability that manipulated single cells can reform entire plants.

Step-by-Step Solution:

Verification / Alternative check:

Standard plant transformation manuals emphasize establishing embryogenic callus/suspensions prior to protoplast isolation to ensure post-manipulation recovery of plants, confirming the dependency.

Why Other Options Are Wrong:

Detection assays (blots, gels) are molecular analyses and do not involve plant regeneration. Microinjection options refer to animal cells, not plants.

Common Pitfalls:

Confusing molecular diagnostics with regeneration-dependent techniques. Only cell-to-plant workflows critically depend on embryogenic suspensions.

Final Answer:

Protoplast culture and whole-plant regeneration