Discussion
Home ‣ Biochemical Engineering ‣ Immobilized Enzyme Comments
- Question
To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, ? can be defined as
Options- A. reaction rate/rate if not slowed by diffusion
- B. rate if not slowed by diffusion/ reaction rate
- C. actual reaction rate/ rate if not slowed by diffusion
- D. rate if not slowed by diffusion/ actual reaction rate
- Correct Answer
- actual reaction rate/ rate if not slowed by diffusion
- 1. For a steady state condition, the change of substrate concentration (dCs/dt) is
Options- A. zero
- B. 1
- C. >1
- D. <1 Discuss
- 2. Commonly employed water insoluble supports for the covalent attachment of enzyme include
Options- A. acrylamide based polymers
- B. polypeptides
- C. dextran
- D. all of these Discuss
- 3. The immobilization technique involving physical method is
Options- A. covalent bond formation dependent
- B. non-covalent bond formation dependent
- C. both (a) and (b)
- D. ionic bond formation dependent Discuss
- 4. Which of the following is not the correct answer?
Options- A. Immobilized enzyme may show selectively altered chemical properties
- B. Immobilized enzyme may show selectively altered physical properties
- C. Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell
- D. None of the above Discuss
- 5. If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
Options- A. rp = ksa(Csb-Cs)
- B. rp = ksaCsb
- C. rp = ksaCs
- D. rp = ksa(Csb+Cs) Discuss
- 6. A fed-batch reactor containing 1 g.l-1 of substrate in 2 litre of medium. It is fed with 1 g.l-l of substrate at the rate of 1 litre per hour. After 10 hours, the concentration of substrate in the reactor was 0.5 g.l-1 . The mass of substrate that was used by the culture in the reactor was
Options- A. 2g
- B. 6g
- C. 8g
- D. 12g Discuss
- 7. A continuous reactor at steady state contains 0.04 g.l-1 of biomass and 0.02 g.l-1 of phenol. The feed contained 0.1 g.l-1 of phenol. The biomass yield would be
Options- A. 0.4 g.g-1
- B. 0.5 g.g-1
- C. 1.0 g.g-1
- D. 5.0 g.g-1 Discuss
- 8. In a continuous reactor, the medium contains 40 g.l-1 of maltose and the medium flow rate is 10 litres per hour and the effluent contains 20 g.l-l of lactate. What is the productivity of lactate production from this reactor?
Options- A. 50 g maltose.l-1
- B. 50 g lactate.l-1
- C. 200 g maltose.l-1
- D. 200 g lactate.l-1 Discuss
- 9. On a glucose medium, the growth of an organism is characterized by the following Monod model and stoichiometric parameters ?m = 0.3 h-1, Ks =0.05 g.l-1 and Yxs = 0.3 g.g-1 When this organism is grown in a 2 litre continuous on a medium containing 10 g.l-1 of glucose added at 1 l.h-1, the steady state concentration of biomass in the reactor will be
Options- A. 0 g.l-1
- B. 0.4 g.l-1
- C. 0.5 g.l-1
- D. 10 g.l-1 Discuss
- 10. Which of the following would not be subjected to the "glucose effect"?
Options- A. Aspergillus fumigatus
- B. Saccharomyces cerevisiae
- C. Escherichia coli
- D. Aspergillus niger Discuss
Immobilized Enzyme problems
Search Results
Correct Answer: zero
Correct Answer: all of these
Correct Answer: non-covalent bond formation dependent
Correct Answer: None of the above
Correct Answer: rp = ksaCsb
Correct Answer: 6g
Correct Answer: 0.5 g.g-1
Correct Answer: 200 g lactate.l-1
Correct Answer: 0 g.l-1
Correct Answer: Escherichia coli
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