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Home ‣ Biochemical Engineering ‣ Immobilized Enzyme Comments
- Question
The covalent attachment of enzyme molecules is via
Options- A. nonessential amino acids residues to water insoluble, functional supports
- B. essential amino acids residues to water insoluble, functional supports
- C. nonessential amino acids residues to water soluble, functional supports
- D. essential amino acids residues to water soluble, functional supports
- Correct Answer
- nonessential amino acids residues to water insoluble, functional supports
- 1. Which of the following is taken as an assumption in the distributed model?
Options- A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
- B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
- C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
- D. All of the above Discuss
- 2. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options- A. free ?-, ?- or ? carb oxyl groups
- B. ? or ? amino groups
- C. phenyl, hydroxyl, sulfhydryl or imidazole groups
- D. all of the above Discuss
- 3. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
Options- A. equal to that of substrate consumption
- B. more than that of substrate consumption
- C. lesser than that of substrate consumption
- D. is nothing to do with the substrate consumption Discuss
- 4. The immobilized technique involving chemical method is
Options- A. covalent bond formation dependent
- B. non-covalent bond formation dependent
- C. both (a) and (b)
- D. ionic bond formation dependent Discuss
- 5. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
Options- A. low molecular weight
- B. high molecular weight
- C. high equivalent weight
- D. low reactivity Discuss
- 6. For glucose isomerization by immobilized enzyme, the reactor generally used is
Options- A. CSTR
- B. plug flow
- C. packed bed
- D. fluidized bed Discuss
- 7. The effectiveness factor increases with the
Options- A. increase of diffusivity and decreased with the increase of particle size
- B. increase of diffusivity and increased with the increase of particle size
- C. decrease of diffusivity and decreased with the increase of particle size
- D. increase of diffusivity and decrease with the decrease of particle size Discuss
- 8. The most commonly employed cross-linked polymer is the
Options- A. polyacrylamide gel
- B. collagen
- C. celluloses
- D. cation exchange resin Discuss
- 9. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options- A. It is possible to separate and purify the enzymes while being immobilized
- B. The enzymes are not usually deactivated by adsorption
- C. The adsorption is a reversible process
- D. State of immobilization is very sensitive to solution pH, ionic strength and temperature Discuss
- 10. The immobilized enzyme produced by micro encapsulation technique provides
Options- A. an extremely large surface area
- B. smaller surface area
- C. high amount of solvent
- D. relatively smaller surface area Discuss
Immobilized Enzyme problems
Search Results
Correct Answer: All of the above
Correct Answer: all of the above
Correct Answer: equal to that of substrate consumption
Correct Answer: covalent bond formation dependent
Correct Answer: low molecular weight
Correct Answer: packed bed
Correct Answer: increase of diffusivity and decreased with the increase of particle size
Correct Answer: polyacrylamide gel
Correct Answer: State of immobilization is very sensitive to solution pH, ionic strength and temperature
Correct Answer: an extremely large surface area
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