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Home Biochemical Engineering Immobilized Enzyme Comments

  • Question
  • The immobilized technique involving chemical method is


  • Options
  • A. covalent bond formation dependent
  • B. non-covalent bond formation dependent
  • C. both (a) and (b)
  • D. ionic bond formation dependent

  • Correct Answer
  • covalent bond formation dependent 


  • Immobilized Enzyme problems


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    • 1. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

    • Options
    • A. low molecular weight
    • B. high molecular weight
    • C. high equivalent weight
    • D. low reactivity
    • Discuss
    • 2. The support material for immobilization of cells of Bacillus subtilis is

    • Options
    • A. ion exchange resins
    • B. gelatin
    • C. Anthracite
    • D. agarose and carbodiimide
    • Discuss
    • 3. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

    • Options
    • A. copolymerization or microencapsulation
    • B. crosslinking using multifunctional reagents
    • C. adsorption
    • D. all of the above
    • Discuss
    • 4. Which is not the method for producing immobilized enzymes with multifunctional reagents?

    • Options
    • A. Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
    • B. Functional groups are introduced on the support to react co-valently with enzymes
    • C. Enzymes are cross linked intermolecularly
    • D. None of the above
    • Discuss
    • 5. The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)

    • Options
    • A. Y = YmaxX/(KL+X)
    • B. Y = Ymax/(KL+X)
    • C. Y = YmaxX/KL
    • D. Y = Ymax- X/ (KL+X)
    • Discuss
    • 6. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

    • Options
    • A. equal to that of substrate consumption
    • B. more than that of substrate consumption
    • C. lesser than that of substrate consumption
    • D. is nothing to do with the substrate consumption
    • Discuss
    • 7. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are

    • Options
    • A. free ?-, ?- or ? carb oxyl groups
    • B. ? or ? amino groups
    • C. phenyl, hydroxyl, sulfhydryl or imidazole groups
    • D. all of the above
    • Discuss
    • 8. Which of the following is taken as an assumption in the distributed model?

    • Options
    • A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
    • B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
    • C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
    • D. All of the above
    • Discuss
    • 9. The covalent attachment of enzyme molecules is via

    • Options
    • A. nonessential amino acids residues to water insoluble, functional supports
    • B. essential amino acids residues to water insoluble, functional supports
    • C. nonessential amino acids residues to water soluble, functional supports
    • D. essential amino acids residues to water soluble, functional supports
    • Discuss
    • 10. For glucose isomerization by immobilized enzyme, the reactor generally used is

    • Options
    • A. CSTR
    • B. plug flow
    • C. packed bed
    • D. fluidized bed
    • Discuss


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