Encapsulation matrices for producing synthetic seeds (e.g., sodium alginate–CaCl2 gels) are typically:
-
ASoluble in water
-
BSoluble in organic solvents
-
CInsoluble in water (hydrogel network that retains integrity)
-
DInsoluble in organic solvents only
-
EVolatile at room temperature
Answer
Correct Answer: Insoluble in water (hydrogel network that retains integrity)
Explanation
Introduction / Context:Encapsulation protects somatic embryos and allows handling similar to seeds. The physical chemistry of the gel determines water retention, gas exchange, and mechanical strength of synthetic seeds.
Given Data / Assumptions:
- Common hydrogels include alginate crosslinked with calcium, carrageenan, and other polysaccharides.
- Gels must be stable in aqueous environments yet permeable to water and solutes.
- Somatic embryos are living and require moisture and oxygen.
Concept / Approach:Calcium alginate forms a crosslinked polymer network that is insoluble in water but swells to form a hydrogel. This provides mechanical protection and a hydrated microenvironment while preventing the bead from dissolving during handling or sowing.
Step-by-Step Solution:
Identify the desired property: maintain bead integrity in moist media.Select material behavior: water-insoluble but water-swollen hydrogel.Therefore the correct description is “insoluble in water.”Verification / Alternative check:Standard encapsulation protocols drop alginate–embryo suspensions into CaCl2 baths to instantly form insoluble gel beads that are then rinsed and stored.
Why Other Options Are Wrong:
- A: If soluble, beads would dissolve during sowing or culture.
- B/D/E: Not representative of hydrogel behavior required for synthetic seeds.
Common Pitfalls:Confusing swelling with solubility; hydrogels can absorb water while remaining insoluble.
Final Answer:Insoluble in water (hydrogel network that retains integrity)