Encapsulation matrices for producing synthetic seeds (e.g., sodium alginate–CaCl2 gels) are typically:

Biotechnology Synthetic Seeds Difficulty: Easy
Choose an option
  • A
    Soluble in water
  • B
    Soluble in organic solvents
  • C
    Insoluble in water (hydrogel network that retains integrity)
  • D
    Insoluble in organic solvents only
  • E
    Volatile at room temperature

Answer

Correct Answer: Insoluble in water (hydrogel network that retains integrity)

Explanation

Introduction / Context:Encapsulation protects somatic embryos and allows handling similar to seeds. The physical chemistry of the gel determines water retention, gas exchange, and mechanical strength of synthetic seeds.

Given Data / Assumptions:

  • Common hydrogels include alginate crosslinked with calcium, carrageenan, and other polysaccharides.
  • Gels must be stable in aqueous environments yet permeable to water and solutes.
  • Somatic embryos are living and require moisture and oxygen.

Concept / Approach:Calcium alginate forms a crosslinked polymer network that is insoluble in water but swells to form a hydrogel. This provides mechanical protection and a hydrated microenvironment while preventing the bead from dissolving during handling or sowing.

Step-by-Step Solution:

Identify the desired property: maintain bead integrity in moist media.Select material behavior: water-insoluble but water-swollen hydrogel.Therefore the correct description is “insoluble in water.”

Verification / Alternative check:Standard encapsulation protocols drop alginate–embryo suspensions into CaCl2 baths to instantly form insoluble gel beads that are then rinsed and stored.

Why Other Options Are Wrong:

  • A: If soluble, beads would dissolve during sowing or culture.
  • B/D/E: Not representative of hydrogel behavior required for synthetic seeds.

Common Pitfalls:Confusing swelling with solubility; hydrogels can absorb water while remaining insoluble.

Final Answer:Insoluble in water (hydrogel network that retains integrity)

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