Question 1

At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

Accepted Answer

oxidative pathway

Question 2

Toxicity due to accumulation of ammonia can be overcome

Accepted Answer

all of the above

Question 3

Which of the following is incorrect?

Accepted Answer

ECL grow in higher density

Question 4

The technique of organ culture may be divided on the basis of employing

Accepted Answer

both (a) and (b)

Question 5

According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

Accepted Answer

12 amino acids

Question 6

What is the concentration of CO2 required for culturing animal cells?

Accepted Answer

1-10%

Question 7

Accumulation of lactate leads to

Accepted Answer

reduction in the pH of culture hence loss of cell viability

Question 8

Which of the following is the technique used for the embryo culture?

Accepted Answer

All of these

Question 9

To prevent the accumulation of lactate

Accepted Answer

low glucose concentration is required

Question 10

How does CO2 help in the cell metabolism during cell culture?

Accepted Answer

It participates in the de novo synthesis of purines and pyrimidines

Question 11

What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Accepted Answer

Lactate helps in the growth while ammonium inhibits the growth

Question 12

Higher dissolved oxygen concentration in the culture media are toxic and lead to

Accepted Answer

all of the above

Question 13

pH of culture medium is initially controlled by

Accepted Answer

presence of bicarbonate buffer

Question 14

Disaggregating of cells can be achieved by

Accepted Answer

all of the above

Animal Cell Culture and Regulation Questions