Colony morphology on Löwenstein–Jensen (LJ) medium: Which mycobacterial species classically produces rough, tough, and buff (nonpigmented, wrinkled) colonies on LJ medium during culture?

Introduction / Context:
Colony morphology on Löwenstein–Jensen medium provides classic visual clues in tuberculosis laboratories. The phrase “rough, tough, and buff” is a time-honored mnemonic for a particular organism’s appearance in culture.

Given Data / Assumptions:

• LJ medium supports growth of slow-growing mycobacteria.
• “Rough, tough, buff” describes colony texture (wrinkled, resilient) and color (buff, nonchromogenic).
• Multiple mycobacteria can be cultured, but not all show this hallmark morphology.

Concept / Approach:
Mycobacterium tuberculosis forms rough, wrinkled, nonchromogenic colonies on LJ medium, often described as “rough, tough, and buff.” Mycobacterium bovis tends to form smaller, more dysgonic colonies; rapid growers such as Mycobacterium fortuitum and Mycobacterium chelonae produce colonies more quickly and with different textures and pigmentation patterns. Therefore, the textbook association belongs to M. tuberculosis.

Step-by-Step Solution:
Recall the mnemonic linking rough-tough-buff to M. tuberculosis. Exclude species with distinct colony characteristics or growth rates. Select Mycobacterium tuberculosis as the correct match. Corroborate with cord factor presence and niacin test as complementary clues.

Verification / Alternative check:
Culture atlases consistently photograph M. tuberculosis colonies exhibiting the classic rough, buff appearance on LJ slants.

Why Other Options Are Wrong:
M. bovis colonies differ in size and are more fastidious; rapid growers yield faster, different-appearing colonies; MAC organisms are often smooth and nonchromogenic but lack the classic R/T/B description.

Common Pitfalls:
Assuming all slow growers look the same; subtle but reliable differences exist and support preliminary identification.

Final Answer:
Mycobacterium tuberculosis.