Minimum criteria for a microarray device To qualify as a microarray, the analytical device must satisfy which set of attributes?
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AOrdered spots only; size is irrelevant
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BMicroscopic features only; layout and specificity optional
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CPlanar format only; binding can be nonspecific
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DAll of these (ordered, microscopic, planar, and specific)
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EOnly requires fluorescence detection, regardless of format
Answer
Correct Answer: All of these (ordered, microscopic, planar, and specific)
Explanation
Introduction / Context:Microarrays rely on many miniature test sites arranged in an ordered pattern on a planar support, enabling simultaneous specific binding events and readout.
Given Data / Assumptions:
- “Micro” implies microscopic feature size.
- Arrays require an ordered layout to map signals to probes.
- Planar substrates (glass, silicon) are standard.
- Specific recognition is essential for meaningful analytics.
Concept / Approach:All listed attributes—order, micro-scale features, planar geometry, and specificity—are fundamental. Lacking any one compromises the definition or utility of a microarray device.
Step-by-Step Solution:
Assess each attribute relative to core definitions.Recognize that “all of these” correctly bundles the minimal criteria.Verification / Alternative check:Manufacturers and standards documents specify planar substrates with ordered spots of micrometer scale and validated specific binding chemistries.
Why Other Options Are Wrong:
- Single-attribute choices are incomplete.
- Fluorescence detection is common but not definitional; label-free arrays exist.
Common Pitfalls:Equating detection method with platform definition; the geometry and specificity define microarrays.
Final Answer:All of these (ordered, microscopic, planar, and specific)