Definition of a microarray platform A microarray is an ordered array of microscopic elements on a planar substrate that enables specific binding of which target class?

Difficulty: Easy

Correct Answer: Genes or gene products (nucleic acids or proteins) via complementary/specific interactions

Explanation:


Introduction / Context:
Microarrays miniaturize many binding assays onto a single planar chip, enabling high-throughput analysis of gene expression, genotyping, and protein interactions.



Given Data / Assumptions:

  • “Ordered,” “microscopic,” and “planar” describe the physical layout.
  • “Specific binding” is through complementary base pairing (DNA/RNA) or affinity (antibody–antigen).


Concept / Approach:
DNA microarrays bind nucleic acids via hybridization; protein microarrays bind proteins via affinity capture. While many loci are probed, they do not bind an entire intact genome in one spot as a single binding event.



Step-by-Step Solution:

Identify true scope: macromolecular targets like cDNAs, oligos, or proteins.Exclude “whole genome” as a single binding target; microarrays query many discrete features.Choose the option highlighting genes or gene products.


Verification / Alternative check:
Standard texts depict arrays of thousands of probes each capturing complementary sequences or antigens.



Why Other Options Are Wrong:

  • Whole genome as one entity is not how arrays function; they survey many fragments/loci.
  • Both (a) and (b): false due to (b).
  • None/only small molecules: contradictory to microarray practice.


Common Pitfalls:
Assuming “genome-wide” means a single whole-genome binding event rather than thousands of parallel specific interactions.



Final Answer:
Genes or gene products (nucleic acids or proteins) via complementary/specific interactions

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