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Home Biochemical Engineering Agitation and Aeration Comments

  • Question
  • The maximum solubility of the alkane in the medium is 5 ppm. If the actual dissolved concentration of the alkane in the medium is 1 ppm. what conclusion may be drawn?


  • Options
  • A. The growth rate is limited by the rate of mass transfer of the alkane into the fermentation medium
  • B. The growth rate is limited by the intrinsic kinetic properties of the cells and not by mass transfer
  • C. The growth rate is neither mass transfer nor kinetically limited
  • D. No conclusion on the growth rate can be withdrawn

  • Correct Answer
  • No conclusion on the growth rate can be withdrawn 


  • Agitation and Aeration problems


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    • 1. In the design of a paddle agitator, the ratio of paddle diameter to tank diameter is normally taken as

    • Options
    • A. 0.1
    • B. 0.8
    • C. 0.25
    • D. 0.5
    • Discuss
    • 2. Higher temperatures affect oxygen transfer rates by

    • Options
    • A. increasing KLa but lowering C*o
    • B. lowering KLa but increasing C*o
    • C. increasing KLa and increasing Co
    • D. lowering KLa and lowering Co
    • Discuss
    • 3. Which of the following model holds correct for the two-film theory? (where KL is the mass transfer coefficient, DAB diffusivity and Zf film thickness)

    • Options
    • A. KL = DAB/Zf
    • B. KL = DAB
    • C. KL = Zf/DAB
    • D. KL = DAB.Zf
    • Discuss
    • 4. A microbial population in a suspension culture will only be limited by oxygen availability if the dissolved oxygen

    • Options
    • A. concentration is less than the critical concentration
    • B. concentration is greater than the critical concentration
    • C. levels are greater than the saturation concentration of oxygen
    • D. levels are equal to saturation oxygen level
    • Discuss
    • 5. Small propellers turn at the speed of either

    • Options
    • A. 20 or 150 rpm
    • B. 150 or 300 rpm
    • C. 400 or 800 rpm
    • D. 1150 rpm or 1750 rpm
    • Discuss
    • 6. The power required by an impeller in a gas sparged system compare to the power required by impeller operating at same speed in a gas free liquid is usually

    • Options
    • A. lesser
    • B. higher
    • C. same
    • D. may be lesser or higher depending upon the geometry
    • Discuss
    • 7. The oxygen absorption rate can be determined by

    • Options
    • A. sodium sulfite oxidation method
    • B. dynamic gassing out technique
    • C. direct measurement
    • D. all of these
    • Discuss
    • 8. Which of the following is not classified as an axial flow impeller?

    • Options
    • A. A propeller
    • B. A marine impeller
    • C. A Rushton turbine
    • D. A hydrofoil impeller
    • Discuss
    • 9. The Intermig impeller is

    • Options
    • A. an axial flow impeller which can be used for the culture of aerobic organisms
    • B. directed upwards rather than towards the base of the reactor
    • C. a fork shaped extensions to the impeller facilitate bubble break up
    • D. All of the above
    • Discuss
    • 10. To increase the oxygen transfer rate in a shake flask culture system, which of the following (s) is/are followed?

    • Options
    • A. Liquid volume in the flask is reduced
    • B. Stirrer speed is increased
    • C. Baffled flasks are used
    • D. All of the above
    • Discuss


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