2. In a glucose-yeast extract medium, Lactococcus lactis has a maximum specific growth rate (um) of 1.23 h-1. What would be the specific growth rate of this organism at steady state in a 4 litre reactor being fed at 2 litres per hour?
3. Two continuous bioreactors containing the same organisms, fed with the same feed at the same dilution rate were compared. Reactor 1 started with an initial concentration of glucose of 10 g.l-1, while reactor 2 contained 0.1 g.l-1 of glucose at the start of the process then at steady state
Options
A. the concentration of glucose in reactor 1 would be greater than that in reactor 2
B. the concentration of glucose in reactor 1 would be equal to reactor 2
C. the concentration of glucose in reactor 1 would always be zero.
D. the concentration of glucose in reactor 1 would be less than that in reactor 2
6. Production of organic acids in batch culture is generally growth associated phase followed by non-growth associated production. The probable reason for this is that
Options
A. organic acids uncouple catabolism from anabolism
B. biomass yields increase as the fermentation proceeds
C. organic acids are secondary metabolites
D. high concentrations of organic acid promote the growth of the microbial population
8. Continuous culture has an advantage over to a batch cultures as
Options
A. continuous reactors do not need to be shut down and cleaned as regularly as a batch reactor
B. most downstream and upstream processes are continuous in nature and thus the operation of continuous bioreactor can be better integrated into the overall manufacturing process
C. cells can be maintained at a constant physiological state simply by setting the dilution rate